NDLI: Metabolic engineering of isoflavone genistein in Brassica napus with soybean isoflavone synthase

Content Provider	Springer Nature Link
Author	Li, Xiang Qin, Jian Chun Wang, Qing Yu Wu, Xian Lang, Chen Yong Pan, Hong Yu Gruber, Margaret Y. Gao, Ming Jun
Copyright Year	2011
Abstract	Genistein, 4′,5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica napus is a non-legume oilseed crop with vegetative tissues producing phenylpropanoids and flavonoids, but does not naturally accumulate isoflavones due to the absence of IFS. To demonstrate whether exogenous IFS is able to use endogenous substrate to produce isoflavone genistein in oilseed crop, the soybean IFS gene (GmIFS2) was incorporated into B. napus plants. The presence of GmIFS2 in B. napus was shown to direct the synthesis and accumulation of genistein derivatives in leaves up to 0.72 mg g−1 DW. In addition, expression levels for most B. napus genes in the phenylpropanoid pathway were altered. These results suggest that the heterologous GmIFS2 enzyme is functionally active at using the B. napus naringenin as a substrate to produce genistein in oilseed rape.
Starting Page	1435
Ending Page	1442
Page Count	8
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	30
Issue Number	8
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-03-16
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Metabolic engineering Brassica napus Genistein GmIFS2 Biotechnology Plant Sciences Plant Biochemistry Cell Biology
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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