NDLI: Transgenic Campanula carpatica plants with reduced ethylene sensitivity

Content Provider	Springer Nature Link
Author	Sriskandarajah, Sridevy Mibus, Heiko Serek, Margrethe
Copyright Year	2006
Abstract	Fertile transgenic Campanula carpatica Jacq. plants with flowers, which had reduced sensitivity to ethylene were obtained by Agrobacterium tumefaciens that mediated transformation. The construct used for transformation contained the etr1-1 gene from Arabidopsis thaliana under control of the flower specific fbp1-promoter from petunia. More than 100 flowering T0 lines were tested for their ethylene sensitivity using 2 μl l−1 ethylene. The tolerance level to ethylene varied among the lines. While control plants stopped flowering within 3 days of exposure to ethylene, one of the transformed lines flowered for up to 27 days. The presence and the expression pattern of the transgene in various tissues were studied by polymerase chain reaction (PCR) and reverse transcription (RT)-PCR techniques. The expression of etr1-1 was significant in flowers and buds. Transgenic lines did not differ morphologically from control plants. The selected transgenic T0 lines, which were re-established from in vitro cultures showed the same degree of tolerance to exogenous ethylene, confirming the stability of the transgene in in vitro cultures. The rooting ability of the transgenic plants was not affected by the presence of etr1-1. T1 progeny were produced by crossing the transgenic line, which showed the most significant reduction in ethylene sensitivity with a control plant, and the analysis of the T1 plants showed 1:1 segregation in terms of ethylene sensitivity and the presence of the transgene.
Starting Page	805
Ending Page	813
Page Count	9
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	26
Issue Number	6
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-01-13
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Agrobacterium tumefaciens Campanula carpatica etr1-1 Flower longevity Flower specific promoter—fbp1 Inheritance Plant Biochemistry Biotechnology Plant Sciences Cell Biology
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Kalanchoe blossfeldiana plants expressing the Arabidopsis etr1-1 allele show reduced ethylene sensitivity

Agrobacterium tumefaciens-mediated transformation of Campanula carpatica: factors affecting transformation and regeneration of transgenic shoots

Improved Postharvest Quality of Inflorescences of fbp1::etr1-1 Transgenic Burrageara ‘Stefan Isler Lava Flow’

Regeneration and transformation in adult plants of Campanula species

The pollen- and embryo-specific Arabidopsis DLL promoter bears good potential for application in marker-free Cre/loxP self-excision strategy

Agrobacterium tumefaciens-mediated genetic transformation of a recalcitrant grain legume, lentil (Lens culinaris Medik)

Generation of transgenic Lolium temulentum plants by Agrobacterium tumefaciens-mediated transformation

Okadaic acid and trifluoperazine enhance Agrobacterium-mediated transformation in eastern white pine

Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance

Transgenic Campanula carpatica plants with reduced ethylene sensitivity

Similar Documents

Kalanchoe blossfeldiana plants expressing the Arabidopsis etr1-1 allele show reduced ethylene sensitivity

Agrobacterium tumefaciens-mediated transformation of Campanula carpatica: factors affecting transformation and regeneration of transgenic shoots

Improved Postharvest Quality of Inflorescences of fbp1::etr1-1 Transgenic Burrageara ‘Stefan Isler Lava Flow’

Regeneration and transformation in adult plants of Campanula species

The pollen- and embryo-specific Arabidopsis DLL promoter bears good potential for application in marker-free Cre/loxP self-excision strategy

Agrobacterium tumefaciens-mediated genetic transformation of a recalcitrant grain legume, lentil (Lens culinaris Medik)

Generation of transgenic Lolium temulentum plants by Agrobacterium tumefaciens-mediated transformation

Okadaic acid and trifluoperazine enhance Agrobacterium-mediated transformation in eastern white pine

Silencing Agrobacterium oncogenes in transgenic grapevine results in strain-specific crown gall resistance

Transgenic Campanula carpatica plants with reduced ethylene sensitivity