NDLI: Structural and functional profile of the carbohydrate esterase gene complement in Phytophthora infestans

Content Provider	Springer Nature Link
Author	Ospina Giraldo, Manuel D. McWalters, Jessica Seyer, Lauren
Copyright Year	2010
Abstract	The plant cell cuticle is the first obstacle for penetration of the host by plant pathogens. To breach this barrier, most pathogenic fungi employ a complex assortment of cell wall-degrading enzymes including carbohydrate esterases, glycoside hydrolases, and polysaccharide lyases. We characterized the full complement of carbohydrate esterase-coding genes in three Phytophthora species and analyzed the expression of cutinase in vitro and in planta; we also determined the cutinase allele distribution in multiple isolates of P. infestans. Our investigations revealed that there are 49, 21, and 37 esterase homologs in the P. infestans, P. ramorum, and P. sojae genomes, respectively, with a considerable number predicted to be extracellular. Four cutinase gene copies were found in both the P. infestans and P. ramorum genomes, while 16 copies were found in P. sojae. Transcriptional analyses of cutinase in P. infestans revealed that its expression level during infection is significantly upregulated at all time points compared to that of the same gene in mycelium grown in vitro. Expression achieves maximum values at 15 hpi, declining at subsequent time points. These results may suggest, therefore, that cutinase most likely plays a role in P. infestans pathogenicity.
Starting Page	495
Ending Page	506
Page Count	12
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	56
Issue Number	6
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-08-20
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phytophthora Cell-wall degrading enzymes Carbohydrate esterases Cutinase Pathogenicity Proteomics Plant Sciences Cell Biology Biochemistry Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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