NDLI: Characterization of the mitochondrial cytochrome b gene from Venturia inaequalis

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Characterization of the mitochondrial cytochrome b gene from Venturia inaequalis

Content Provider	Springer Nature Link
Author	Zheng, Desen Köller, W.
Copyright Year	1997
Abstract	A new class of agricultural fungicides derived from the group of antifungal strobilurins acts as specific respiration inhibitors by binding to mitochondrial cytochrome b. The cytochrome b gene was cloned and sequenced from the mitochondrial genome of Venturia inaequalis, the causal agent of apple scab. The gene was 10.65 kbp in size and contained seven exons and six introns. The exons encoded a protein of 393 amino acids. Comparison of the deduced amino-acid sequence with cytochrome b proteins from other fungi revealed highest homologies to the respective proteins of Aspergillus nidulans, Podospora anserina and Neurospora crassa. All amino acids of the V. inaequalis cytochrome b at positions altered in mutants of Saccharomyces cerevisiae resistant to strobilurins, and other fungi with reduced sensitivities to strobilurins, were identical to wild-type isolates of several fungi. The cloning and characterization of the V. inaequalis cytochrome b gene is the initial step in the assessment of resistance risks inherent to the strobilurin fungicides.
Starting Page	361
Ending Page	366
Page Count	6
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	32
Issue Number	5
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	1997-11-28
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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