NDLI: Multiple displacement amplification, a powerful tool for molecular genetic analysis of powdery mildew fungi

Content Provider	Springer Nature Link
Author	Fernández Ortuño, Dolores Torés, Juan A. Vicente, Antonio Pérez García, Alejandro
Copyright Year	2007
Abstract	Powdery mildew fungi (Erysiphales) are probably the largest group of plant pathogens that remain uncharacterized from genetic and molecular points of view, with the only exception of the powdery mildew of cereals, Blumeria graminis. Their nature as obligate biotrophic parasites and consequent inability to grow on culture media has significantly hampered research. A common bottleneck to the molecular genetic analysis of powdery mildew fungi is the availability of genomic DNA of suitable quality and in sufficient quantity. The so-called whole genome amplification technology has the potential to overcome this limitation. Here we present the application of phi29 DNA polymerase-mediated multiple displacement amplification (MDA) to amplify the whole genome of Podosphaera fusca, the main causal agent of powdery mildew in cucurbits, to address this problem. The genome coverage and fidelity of the MDA process was evaluated by PCR amplification and sequencing of two genetics markers: the nuclear rDNA internal transcribed spacer (ITS) regions and the mitochondrial cytochrome b gene (CYTB). Our results show that MDA is a valuable tool for molecular genetic analysis of powdery mildew fungi that can be used for a number of downstream applications in different fields, such as epidemiology and population genetics or systematics.
Starting Page	209
Ending Page	219
Page Count	11
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	51
Issue Number	3
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-01-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cytochrome b ITS regions phi29 DNA polymerase Podosphaera fusca Whole genome amplification Proteomics Plant Sciences Cell Biology Biochemistry Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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