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Influence of gene dosage and autoregulation of the regulatory genes INO2 and INO4 on inositol/choline-repressible gene transcription in the yeast Saccharomyces cerevisiae

The VPS4 gene is involved in protein transport out of a yeast pre-vacuolar endosome-like compartment

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Current Genetics : Volume 31, Issue 5, May 1997

Current Genetics : Volume 31, Issue 4, April 1997

Current Genetics : Volume 31, Issue 3, March 1997

Current Genetics : Volume 31, Issue 2, February 1997

Current Genetics : Volume 31, Issue 1, January 1997

The VPS4 gene is involved in protein transport out of a yeast pre-vacuolar endosome-like compartment

Content Provider	Springer Nature Link
Author	Finken Eigen, Marion Röhricht, Ralf A. Köhrer, K.
Copyright Year	1997
Abstract	Four yeast mutants were isolated in a screen for dominant-negative vacuolar protein-sorting mutants, secreting a carboxypeptidase Y-invertase hybrid protein. In addition to defects in the sorting/transport of soluble vacuolar hydrolases, the mutants accumulated a pre-vacuolar endosome-like compartment. The mutant alleles causing the defects were identified as the members of the VPS4 gene locus, each harbouring single-point mutations leading to amino-acid exchanges at positions 233 (E233Q), 211 (E211 K), and 178 (G178D). These mutations all reside within a 200 amino-acid-long ATPase module, common to members of the AAA-protein family. The VPS4 gene product shows homology to the yeast Sec18p (50% similarity and 25% identity), which is involved in several vesicle-mediated protein transport steps and homotypic membrane fusion events. Disruption of the VPS4 gene leads to a recessive vacuolar protein-sorting phenotype. About 40% of newly synthesized CPY is secreted as the Golgi-modified p2CPY precursor form. Transport of secretory proteins to the plasma membrane is normal as demonstrated by the secretion of invertase and α-factor. The α-factor, however, is secreted as a partially processed precursor, caused by defects in late Golgi function. The vps4 mutants also exhibit defects in fluid-phase endocytosis, as demonstrated by the accumulation of Lucifer Yellow in a pre-vacuolar endosome-like compartment. Based on the pleiotropic phenotype of the vps4 mutants and on the sequence homology to NSF/Sec18p, we propose that the VPS4 gene product is required for efficient transport out of the pre-vacuolar endosome-like compartment.
Starting Page	469
Ending Page	480
Page Count	12
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	31
Issue Number	6
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	1997-07-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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The VPS4 gene is involved in protein transport out of a yeast pre-vacuolar endosome-like compartment

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