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Current Genetics : Volume 35

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Functional domain analysis of the Saccharomyces MAL-activator

Content Provider	Springer Nature Link
Author	Hu, Zhen Gibson, Andrew W. Kim, Jeong H. Wojciechowicz, Lori A. Zhang, Bin Michels, Corinne A.
Copyright Year	1999
Abstract	MAL63 of the MAL6 locus and its homologues at the other MAL loci encode transcription activators required for the maltose-inducible expression of the MAL structural genes. We carried out a deletion analysis of LexA-MAL63 gene fusions to localize the functional domains of the Mal63 MAL-activator protein. Our results indicate that the sequence-specific DNA-binding domain of Mal63p is contained in residues 1–100; that residues 60–283 constitute a functional core region including the transactivation domain; that residues 251–299 are required to inhibit the activation function of Mal63p; and that the rest of the C-terminal region of the protein contains a maltose-responsive domain that acts to relieve the inhibitory effect on the activation function. Abundant overproduction of Mal63p does not overcome the negative regulation of MAL gene expression in the absence of maltose, suggesting that a titratable MAL-specific repressor similar to Gal80p is not involved in the negative regulation of the MAL-activator. A model for maltose-inducible autoregulation of the MAL-activator is presented.
Starting Page	1
Ending Page	12
Page Count	12
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	36
Issue Number	1-2
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-08-17
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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