NDLI: Gene silencing of transgenes inserted in the Aspergillus nidulans alcM and/or alcS loci

Content Provider	Springer Nature Link
Author	Robellet, Xavier Oestreicher, Nathalie Guitton, Audrey Vélot, Christian
Copyright Year	2010
Abstract	While carrying out a systematic disruption of the genes of unknown function in the alc gene cluster from the filamentous fungus Aspergillus nidulans, we observed a strong diminution of the transcription of markers inserted in the alcS gene. This was found to be the case for the two markers tested, nadA (from A. nidulans) and pyrG (from A. fumigatus) involved in purine utilization and uracil/uridine biosynthetic pathway, respectively. The same phenomenon was also observed with insertion of the nadA gene in the alcM locus, another gene of the alc cluster. In the case of nadA, the level of expression was directly correlated to the ability of the corresponding strains to grow on adenine as a sole nitrogen source. The insertion of the pyrG marker into alcS complemented perfectly vegetative growth, but did not allow a proper sexual cycle. This suggests that the lowered pyrG expression is not sufficient to provide the intracellular concentration of pyrimidines required for the sexual cycle. Thus, due caution must be exercised when disrupting genes with pyrG, one of the most commonly employed markers, especially if the gene to be disrupted is involved or suspected to be involved in the sexual cycle.
Starting Page	341
Ending Page	348
Page Count	8
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	56
Issue Number	4
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-05-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aspergillus nidulans Selectable marker genes Gene disruption Sexual development Position effects Proteomics Plant Sciences Cell Biology Biochemistry Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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