NDLI: Roles of Ebp2 and ribosomal protein L36 in ribosome biogenesis in Saccharomyces cerevisiae

Content Provider	Springer Nature Link
Author	Wan, Kun Yabuki, Yukari Mizuta, Keiko
Copyright Year	2014
Abstract	Ebp2 plays an essential role in biogenesis of 60S ribosomal subunits. We determined the genetic interactions between EBP2 and RPL36A/B, which encodes ribosomal protein L36a/b. RPL36A/B was a multicopy suppressor to ebp2 mutants, and the suppression was not common to defects in ribosome biogenesis resulting from other mutations of assembly factors. Disruption of RPL36A or RPL36B caused synthetic enhancement of the growth defect of the ebp2-14 allele at high temperatures. Disruption of RPL36B led to a more severe growth defect than that of RPL36A due to imbalances in the expression levels of the duplicated genes. Primer-extension analysis revealed that L36a/b is required for the processing of 27SA2, 27SA3, and 27SBL pre-rRNAs. Two-hybrid analysis indicated that Ebp2 interacts with ribosomal proteins L36a/b, L34a/b, and L8, which in mature ribosomes are located adjacent to each other in close proximity to the 3′ end of 5.8S rRNA. These results suggest that Ebp2 functions cooperatively with ribosomal proteins L36, L34, and L8 in biogenesis of the 60S ribosomal subunit.
Starting Page	31
Ending Page	41
Page Count	11
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	61
Issue Number	1
e-ISSN	14320983
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-08-14
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ribosome biogenesis EBP2 Ribosomal protein L36a/b Ribosomal protein L34a/b Duplicated genes Microbial Genetics and Genomics Microbiology Biochemistry Cell Biology Plant Sciences Proteomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Multiple roles of the general regulatory factor Abf1 in yeast ribosome biogenesis

Ribosomal protein and biogenesis factors affect multiple steps during movement of the Saccharomyces cerevisiae Ty1 retrotransposon

Functional characterization of ribosomal protein L15 from Saccharomyces cerevisiae

$N^{α}-Acetyltransferase$ NatA Is Involved in Ribosome Synthesis in Saccharomyces cerevisiae

The yeast CPC2/ASC1 gene is regulated by the transcription factors Fhl1p and Ifh1p

Dual role of ribosome-associated chaperones in prion formation and propagation

U3 snoRNA promoter reflects the RNA’s function in ribosome biogenesis

Ste50 adaptor protein influences Ras/cAMP-driven stress-response and cell survival in Saccharomyces cerevisiae

Purifying selection against gene conversions between the polyamine transport (TPO) genes of Saccharomyces species

Roles of Ebp2 and ribosomal protein L36 in ribosome biogenesis in Saccharomyces cerevisiae

Similar Documents

Multiple roles of the general regulatory factor Abf1 in yeast ribosome biogenesis

Ribosomal protein and biogenesis factors affect multiple steps during movement of the Saccharomyces cerevisiae Ty1 retrotransposon

Functional characterization of ribosomal protein L15 from Saccharomyces cerevisiae

$N^{α}-Acetyltransferase$ NatA Is Involved in Ribosome Synthesis in Saccharomyces cerevisiae

The yeast CPC2/ASC1 gene is regulated by the transcription factors Fhl1p and Ifh1p

Dual role of ribosome-associated chaperones in prion formation and propagation

U3 snoRNA promoter reflects the RNA’s function in ribosome biogenesis

Ste50 adaptor protein influences Ras/cAMP-driven stress-response and cell survival in Saccharomyces cerevisiae

Purifying selection against gene conversions between the polyamine transport (TPO) genes of Saccharomyces species

Roles of Ebp2 and ribosomal protein L36 in ribosome biogenesis in Saccharomyces cerevisiae