NDLI: Low-affinity iron transport protein Uvt3277 is important for pathogenesis in the rice false smut fungus Ustilaginoidea virens

Content Provider	Springer Nature Link
Author	Zheng, Meng ting Ding, Hui Huang, Lei Wang, Ya hui Yu, Mi na Zheng, Rui Yu, Jun jie Liu, Yong feng
Copyright Year	2016
Abstract	Ustilaginoidea virens is the causal agent of rice false smut disease resulting in quantitative and qualitative losses in rice. To gain insights into the pathogenic mechanisms of U. virens, we established a T-DNA insertion mutant library of U. virens through Agrobacterium tumefaciens-mediated transformation and selected an enhanced pathogenicity mutant (i.e., B3277). We analyzed the biological characteristics of the wild-type P1 and B3277. The growth rate and sporulation of B3277 were decreased compared with those of P1; the ferrous iron could be utilized by B3277, but inhibited the growth of P1. Southern blot analysis was performed to verify the copy number of the foreign gene inserted in the genomic DNA and only one copy of the T-DNA was found. The combined hiTAIL-PCR with RACE-PCR analysis showed the successful cloning of full length of the T-DNA flanking gene associated with pathogenicity, named Uvt3277. Gene expression was analyzed using real-time PCR. Results revealed that Uvt3277 was expressed at lower levels in B3277 than in P1. This gene was then subjected to bioinformatics analysis. The encoded protein of Uvt3277 exhibited high homology with low-affinity iron transporter proteins in some fungi. Transformation of the RNAi vector by constructing the hairpin RNA of the target gene was confirmed as successful. The pathogenicity of the transformant also increased. These results suggested that Uvt3277 may have an important function associated with the pathogenesis of U. virens. This study provides insights into the pathogenic mechanism of U. virens and a molecular target of disease control.
Starting Page	131
Ending Page	144
Page Count	14
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	63
Issue Number	1
e-ISSN	14320983
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-06-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ustilaginoidea virens T-DNA Pathogenicity Gene clone RNAi Microbial Genetics and Genomics Microbiology Biochemistry Cell Biology Plant Sciences Proteomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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