NDLI: Allele-specific expression of the Mgi– phenotype on disruption of the F1-ATPase delta-subunit gene in Kluyveromyces lactis

Content Provider	Springer Nature Link
Author	Hansbro, P. M. Chen, Xin Jie Clark Walker, G. D.
Copyright Year	1998
Abstract	Kluyveromyces lactis is a petite-negative yeast that does not form viable mitochondrial genome-deletion mutants (petites) when treated with DNA-targeting drugs. Loss of mtDNA is lethal for this yeast but mutations at three loci termed MGI, for mitochondrial genome integrity, can suppress this lethality. The three loci encode the α-, β- and γ-subunits of mitochondrial F1-ATPase. In this study we report the isolation and characterization of the KlATPδ gene encoding the δ-subunit of F1-ATPase. The deduced protein contains 158 amino acids showing 72% identity to the protein from Saccharomyces cerevisiae and a putative mitochondrial targeting sequence of 23 amino acids. Disruption of the gene causes cells to become respiratory deficient while the introduction of ATPδ from S. cerevisiae restores growth on glycerol. Cells with a disrupted ATPδ gene, like strains with disruptions of α-, β- and γ-F1-subunits, do not produce petite mutants when treated with ethidium bromide. However, unlike strains with disruptions in the three largest F1-subunits, disruption of ATPδ in the presence of some mgi alleles does not abolish the Mgi– phenotype. By contrast, elimination of ATPδ in other mgi strains removes resistance to ethidium bromide and ρ 0 mutants are not formed. Hence the ATPδ subunit of F1-ATPase, while not mandatory for a Mgi– phenotype, aids some mgi alleles in suppressing ρ 0 lethality.
Starting Page	46
Ending Page	51
Page Count	6
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	33
Issue Number	1
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-02-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Subject	Genetics Medicine

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