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| Content Provider | Springer Nature Link |
|---|---|
| Author | Cai, Jun Chen, Mingli Wang, Yu Pan, Junfeng Li, Aobo Zhang, Deyuan |
| Copyright Year | 2013 |
| Abstract | We proved the feasibility of using a microfluidic chip to culture diatom Bacillaria paradoxa, and analyzed the gliding characteristics of its self-organized colony in detail. The optimal cultivation parameters of B. paradoxa for the designed chip made with polydimethylsiloxane are as follows: the preferable cells injecting rate for keeping the cells alive is 0.2 mL/h, the initial cell density for fast reproduction is 5.5 × 104 cells/mL, and the optimal replacement period of culture medium is 4 days. B. paradoxa tends to form a colony during their growth, and the colony can glide with a steady period of 29 ± 3 s along its axial direction in a constant stream, the amplitude of the colony will not decay (e.g., 24 μm of two-cell colony at 1.1 mm/s flow rate), and the colony rapidly adjusts its direction of gliding to the direction of water flow. The successful culture of diatoms on a microfluidic platform may be used for biosensing chips and the creation of gasoline-producing diatom solar panels. |
| Starting Page | 652 |
| Ending Page | 658 |
| Page Count | 7 |
| File Format | |
| ISSN | 03438651 |
| Journal | Current Microbiology |
| Volume Number | 67 |
| Issue Number | 6 |
| e-ISSN | 14320991 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2013-06-23 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Microbiology Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Applied Microbiology and Biotechnology |
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