NDLI: Bombyx mori Nucleopolyhedrovirus ORF94, A Novel Late Protein Is Identified to be a Component of ODV Structural Protein

Content Provider	Springer Nature Link
Author	Liang, Guiting Li, Guohui Chen, Keping Yao, Qin Chen, Huiqing Zhou, Yang
Copyright Year	2010
Abstract	Orf94 (Bm94) of Bombyx mori nucleopolyhedrovirus (BmNPV) potentially encodes 424-amino acids with a predicted molecular weight of 49.4 kDa, but its function remains unknown. Blast search results revealed that Bm94 homologues exist in 10 completely sequenced Lepidopteron NPVs with identities ranging from 95 to 37%. Results of our recent study showed that Bm94 was transcribed from 12 to 72 h and the corresponding protein was detected from 24 to 72 h post-infection. Furthermore, Western blot analysis revealed that Bm94 was present in occlusion-derived virus (ODV) and in total protein from BmNPV-infected BmN cells, but not in budded virus. Immunofluoresence analysis revealed that the protein located primarily in the cytoplasm and was also present in the nucleus in the later infection. In conclusion, these results together indicated that Bm94 was a late gene, which distributed both in the cytoplasm and in the nucleus, and was identified to be a component of BmNPV ODV.
Starting Page	190
Ending Page	196
Page Count	7
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	61
Issue Number	3
e-ISSN	14320991
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-02-05
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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