NDLI: Biochemical Characterization of a β-1,3-Glucanase from Trichoderma koningii Induced by Cell Wall of Rhizoctonia solani

Content Provider	Springer Nature Link
Author	Monteiro, Valdirene Neves Ulhoa, Cira José
Copyright Year	2006
Abstract	Trichoderma species are readily isolated from Brazilian cerrado soil by conventional methods and some of them were characterized as Trichoderma koningii. The effect of carbon source on the production of β-1,3-glucanases in the culture filtrates of a specific Trichoderma koningii strain (ALL 13) was investigated. Enzyme activity was detected in all carbon sources tested and only one band of β-1,3-glucanase was detected in non-denaturing PAGE. This enzyme was purified by Sephacryl S-200 gel filtration and Phenyl Sepharose CL 4B chromatography. A typical procedure provided 105-fold purification with 13.4% yield. The molecular weight of the purified enzyme was 75 kDa as estimated by SDS-PAGE. The enzyme hydrolyzed laminarin in an endo-like fashion to form small oligosaccharides and glucose. The Km and Vmax values for β-1,3-glucanase, using laminarin as substrate, were 0.148 mg.mL−1 and 0.159 U.min−1, respectively. The pH optimum for the enzyme was pH 4.6 and maximum activity was obtained at 50°C. Hg2+ inhibited the purified enzyme.
Starting Page	92
Ending Page	96
Page Count	5
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	52
Issue Number	2
e-ISSN	14320991
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-01-31
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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