NDLI: Hierarchical Chromatin Structure of Schizosaccharomyces pombe Revealed by Atomic Force Microscopy

Content Provider	Springer Nature Link
Author	Kobori, Toshiro Yoshi, Tomoyuki Sugiyama, Shigeru Ohtani, Toshio
Copyright Year	2003
Abstract	Many structural studies on higher eukaryotic chromatin have been carried out, but chromatin structure in fungi remains unclear. Schizosaccharomyces pombe has been used for investigations of chromosome function; however, the structural details of S. pombe chromatin have not been clarified owing to its small nucleus. We used atomic force microscopy for nano-scale imaging of chromatin isolated from S. pombe. Topographic images indicated that nuclear chromatin contained at least three hierarchical structures: large-scale chromatin fibers, spherical domains in the fibers, and nodules in the domains. The average diameters of the domain and the nodule were 363 ± 85.2 nm and 46.2 ± 9.30 nm. Each structure comprising the hierarchy was similar to higher eukaryotic chromatin thus far observed, despite definite differences in chromatin organization at the nucleosomal level. The presence of histone H1 suggested that there might be an alternative to compensate for histone H1 lacking in S. pombe.
Starting Page	404
Ending Page	407
Page Count	4
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	47
Issue Number	5
e-ISSN	14320991
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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