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| Content Provider | Springer Nature Link |
|---|---|
| Author | Srinivasan, Sathiyaraj Kim, Jinsoo Kang, Sang Rim Jheong, Weon Hwa Lee, Sang Seob |
| Copyright Year | 2012 |
| Abstract | A Gram-negative, aerobic, short-rod-shaped, non-motile bacterium designated Rs7T, was isolated from peat soil collected from Russia and was characterized to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that the strain Rs7T belongs to the class Betaproteobacteria. The highest degree of sequence similarities were determined to be with Burkholderia tropica Ppe8T (98.4 %), Burkholderia unamae MTI-641T (97.8 %), Burkholderia bannensis E25T (97.7 %), Burkholderia heleia SA41T (97.0 %), and Burkholderia sacchari IPT101T (97.0 %). Chemotaxonomic data revealed that the strain Rs7T possesses ubiquinone Q-8. The polar lipid profile of strain Rs7T contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, and an unknown amino phospholipid. The predominant fatty acids were C16:0, C19:0 cyclo ω8c, and C17:0 cyclo, all of which corroborated the assignment of the strain to the genus Burkholderia. The DNA G+C content was 63.2 mol%. DNA–DNA hybridization experiments showed less than 37.8 % DNA relatedness with closely related type strains, thus confirming separate species status. The results of physiological and biochemical tests allowed phenotypic differentiation of strain Rs7T from the members of the genus Burkholderia. Based on these data, Rs7T (=KEMC 7302-068T = JCM 18069T) should be classified as the type strain for a novel Burkholderia species, for which the name Burkholderia humi sp. nov. is proposed. |
| Starting Page | 300 |
| Ending Page | 305 |
| Page Count | 6 |
| File Format | |
| ISSN | 03438651 |
| Journal | Current Microbiology |
| Volume Number | 66 |
| Issue Number | 3 |
| e-ISSN | 14320991 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-11-30 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Microbiology Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Applied Microbiology and Biotechnology |
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