NDLI: Simple and Rapid Detection of Salmonella by Direct PCR Amplification of Gene fimW

Content Provider	Springer Nature Link
Author	Zhang, Jiang ying Dong, Li wei Ren, Qian Wang, Xiao zhou Yang, Yi Zhou, Wen Zhu, Chun hong Meng, Xia Zhu, Guo qiang
Copyright Year	2014
Abstract	This study established a simple method of specifically detecting Salmonella species by amplifying fimW gene, which was involved in regulating Salmonella type I fimbriae expression. A pair of primers was designed to target and discriminate the 68 Salmonella strains of 23 Salmonella serovars available to us from 12 non-Salmonella strains of five different kinds of bacteria by polymerase chain reaction (PCR) amplification. Results showed that specific DNA fragment with an expected size of 477 bp was successfully amplified from all Salmonella serovars, while no target band was detected in non-Salmonella species. The sensitivity of this PCR-amplifying system reached to 1 pg DNA chromosome and 102 cfu of Salmonella enteritis strain CMCC(B) 50336. The above results demonstrated the method as a simple, sensitive, and specific way for Salmonella detection.
Starting Page	429
Ending Page	435
Page Count	7
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	69
Issue Number	4
e-ISSN	14320991
Language	English
Publisher	Springer US
Publisher Date	2014-05-17
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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Simple and Rapid Detection of Salmonella by Direct PCR Amplification of Gene fimW