NDLI: Insights into Two High Homogenous Genes Involved in Copper Homeostasis in Acidithiobacillus ferrooxidans

Content Provider	Springer Nature Link
Author	Luo, Yanjie Liu, Yuandong Zhang, Chenggui Luo, Hailang Guan, Hao Liao, Hehuan Qiu, Guanzhou Liu, Xueduan
Copyright Year	2008
Abstract	Acidithiobacillus ferrooxidans, an important microorganism in bioleaching industry, has been sequenced recently, and from the annotated information, there are four genes involved in copper homeostasis. Sequence analysis showed that two of them, Afe0329 and Afe0663, were high homologous (94.43% identity). With the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) cloning approach, the differential gene expression of these two high homologous genes in a genome was successfully identified for the first time. In comparison with Afe0663, Afe0329 was highly expressed grown in the medium with copper, and the restriction fragment length polymorphism (RFLP) profile showed that 96% of lanes were products of Afe0329. Analysis of the protein sequence encoded by Afe0329 suggested a conserved domain of P1b3-type ATPase, which is a heavy-metal pump, and, to be unexpected, the molecular modeling revealed that the amino acids determining the type of heavy-metal pumps were responsible for the gate of the copper ion channel in the transmembrane area of the protein. The activity of P1b-type ATPase disrupted in Escherichia coli could be partially rescued by complementation by the plasmid-carrying Afe0329 gene. All of these results suggest that a copper homeostasis mechanism including P-type ATPase is of importance for the survival of this extremophilic microorganism.
Starting Page	274
Ending Page	280
Page Count	7
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	57
Issue Number	4
e-ISSN	14320991
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-07-11
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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