NDLI: PrfA Led to Reduced Biofilm Formation and Contributed to Altered Gene Expression Patterns in Biofilm-Forming Listeria monocytogenes

Content Provider	Springer Nature Link
Author	Luo, Qin Shang, Junli Feng, Xiaoqin Guo, Xinxin Zhang, Liang Zhou, Qingchun
Copyright Year	2013
Abstract	The foodborne pathogen Listeria monocytogenes has the ability to develop biofilm in the food-processing environment, which becomes a major concern for food safety. PrfA, a key transcriptional activator that regulates most of the known listerial virulence gene expression, has been shown to promote L. monocytogenes biofilm formation. In this study, the whole-genome microarray was used to identify differentially expressed genes associated with the putative interaction between biofilm formation and PrfA in L. monocytogenes. Comparative transcriptome analyses indicated that over 21.9 % of the L. monocytogenes EGDe genes (627 out of 2,857 predicted) were altered in their expression of biofilm compared to the planktonic phase. These genes were classified into different functional categories which cover most of the biochemical functions encountered in bacterial cells, indicating that L. monocytogenes biofilm formation is probably controlled by a complex regulation network involved in variable genes required for the different biological pathways. Further comparison of gene expression profiles of biofilms between L. monocytogenes EGDe and its PrfA deletion mutant revealed 185 genes associated with PrfA and biofilm formation. Except for 10 genes, transcription levels of 175 genes were completely opposite between ΔprfA and wild-type during the biofilm formation, i.e., up-regulated genes in ΔprfA were down-regulated in the wild-type strain, and vice versa, indicating that loss of PrfA dramatically altered gene expression patterns in L. monocytogenes biofilm and resulted in reduced ability of the biofilm formation.
Starting Page	372
Ending Page	378
Page Count	7
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	67
Issue Number	3
e-ISSN	14320991
Language	English
Publisher	Springer US
Publisher Date	2013-05-08
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Virulence Regulator PrfA is Essential for Biofilm Formation in Listeria monocytogenes but not in Listeria innocua

A study on the effects of some laboratory-derived genetic mutations on biofilm formation by Listeria monocytogenes

In Vitro Evaluation of Bacteriocins Activity Against Listeria monocytogenes Biofilm Formation

Carbon Catabolite Control is Important for Listeria monocytogenes Biofilm Formation in Response to Nutrient Availability

Biofilm formation by different serological variants of Listeria monocytogenes in association with Bacillus pumilus

Development of a loop-mediated isothermal amplification assay for detecting Listeria monocytogenes prfA in milk

The Regulatory Roles of ncRNA Rli60 in Adaptability of Listeria monocytogenes to Environmental Stress and Biofilm Formation

σ$^{B}$ affects biofilm formation under the dual stress conditions imposed by adding salt and low temperature in Listeria monocytogenes

Development of a biofilm model for Listeria monocytogenes EGD-e

PrfA Led to Reduced Biofilm Formation and Contributed to Altered Gene Expression Patterns in Biofilm-Forming Listeria monocytogenes

Similar Documents

Virulence Regulator PrfA is Essential for Biofilm Formation in Listeria monocytogenes but not in Listeria innocua

A study on the effects of some laboratory-derived genetic mutations on biofilm formation by Listeria monocytogenes

In Vitro Evaluation of Bacteriocins Activity Against Listeria monocytogenes Biofilm Formation

Carbon Catabolite Control is Important for Listeria monocytogenes Biofilm Formation in Response to Nutrient Availability

Biofilm formation by different serological variants of Listeria monocytogenes in association with Bacillus pumilus

Development of a loop-mediated isothermal amplification assay for detecting Listeria monocytogenes prfA in milk

The Regulatory Roles of ncRNA Rli60 in Adaptability of Listeria monocytogenes to Environmental Stress and Biofilm Formation

σ$^{B}$ affects biofilm formation under the dual stress conditions imposed by adding salt and low temperature in Listeria monocytogenes

Development of a biofilm model for Listeria monocytogenes EGD-e

PrfA Led to Reduced Biofilm Formation and Contributed to Altered Gene Expression Patterns in Biofilm-Forming Listeria monocytogenes