NDLI: Crystal Violet and XTT Assays on Staphylococcus aureus Biofilm Quantification

Content Provider	Springer Nature Link
Author	Xu, Zhenbo Liang, Yanrui Lin, Shiqi Chen, Dingqiang Li, Bing Li, Lin Deng, Yang
Copyright Year	2016
Abstract	Staphylococcus aureus (S. Aureus) is a common food-borne pathogenic microorganism. Biofilm formation remains the major obstruction for bacterial elimination. The study aims at providing a basis for determining S. aureus biofilm formation. 257 clinical samples of S. aureus isolates were identified by routine analysis and multiplex PCR detection and found to contain 227 MRSA, 16 MSSA, 11 MRCNS, and 3 MSCNS strains. Two assays for quantification of S. aureus biofilm formation, the crystal violet (CV) assay and the XTT (tetrazolium salt reduction) assay, were optimized, evaluated, and further compared. In CV assay, most isolates formed weak biofilm 74.3 %), while the rest formed moderate biofilm (23.3 %) or strong biofilm (2.3 %). However, most isolates in XTT assay showed weak metabolic activity (77.0 %), while the rest showed moderate metabolic activity (17.9 %) or high metabolic activity (5.1 %). In this study, we found a distinct strain-to-strain dissimilarity in terms of both biomass formation and metabolic activity, and it was concluded from this study that two assays were mutual complementation rather than being comparison.
Starting Page	474
Ending Page	482
Page Count	9
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	73
Issue Number	4
e-ISSN	14320991
Language	English
Publisher	Springer US
Publisher Date	2016-06-21
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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