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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ohgushi, Akira Saitoh, Hiroyuki Wasa, Naoya Uemori, Akiko Ohba, Michio |
| Copyright Year | 2005 |
| Abstract | Two new crystal protein genes, cry24B and s1orf2, were cloned from a mosquitocidal Bacillus thuringiensis serovar sotto strain. The cry24B and s1orf2 genes encoded a 76-kDa and 62-kDa protein, respectively. The Cry24B protein retained five conserved regions commonly found in the existing Cry proteins. The amino acid sequence of the S1ORF2 had a high homology to that of the ORF2 protein of B. thuringiensis serovar jegathesan. Southern hybridization experiments with a cry24B gene-specific probe revealed that these genes are located on two large plasmids of > 100 kb. When the two genes, cry24B and s1orf2, were expressed in an acrystalliferous B. thuringiensis host, the proteins were synthesized and accumulated as inclusions. These inclusions exhibited no larvicidal activities against three mosquito species: Aedes aegypti, Anopheles stephensi, and Culex pipiens molestus. Likewise, the inclusions contained no cytocidal activity against HeLa cells. |
| Starting Page | 131 |
| Ending Page | 136 |
| Page Count | 6 |
| File Format | |
| ISSN | 03438651 |
| Journal | Current Microbiology |
| Volume Number | 51 |
| Issue Number | 2 |
| e-ISSN | 14320991 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2005-07-13 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biotechnology Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Applied Microbiology and Biotechnology |
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