NDLI: A Novel Two-Component System amrB-amkB Involved in the Regulation of Central Carbohydrate Metabolism in Rifamycin SV-Producing Amycolatopsis mediterranei U32

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A Novel Two-Component System amrB-amkB Involved in the Regulation of Central Carbohydrate Metabolism in Rifamycin SV-Producing Amycolatopsis mediterranei U32

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A Novel Two-Component System amrB-amkB Involved in the Regulation of Central Carbohydrate Metabolism in Rifamycin SV-Producing Amycolatopsis mediterranei U32

Content Provider	Springer Nature Link
Author	Wang, Weiwu Gao, Jin Chiao, Juishen Zhao, Guoping Jiang, Weihong
Copyright Year	2004
Abstract	A novel two-component signal transduction system amrB-amkB was cloned from rifamycin SV-producing Amycolatopsis mediterranei U32, and their biochemical functions as a response regulator and a histidine protein kinase, respectively, were proven. The amrB disruption mutant was generated by insertional inactivation with the aparmycin resistance gene. The metabolic response to the absence of amrB gene was determined by a biochemical profiling technique in which the concentration changes of metabolic intermediates were measured by gas chromatography with time-of-flight mass spectrometry (GC/TOF-MS). Although the phenotype analyses of the amrB gene disruption mutant showed no significant change with respect to rifamycin SV production and morphological differentiation, the global metabolomic analyses found the concentration levels of some key intermediates in the TCA cycle and glycolysis pathway were affected by an amrB gene disruption event. The primary results suggested that amrB-amkB genes might be involved in the regulation of central carbohydrate metabolism in A. mediterranei U32.
Starting Page	14
Ending Page	19
Page Count	6
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	48
Issue Number	1
e-ISSN	14320991
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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A Novel Two-Component System amrB-amkB Involved in the Regulation of Central Carbohydrate Metabolism in Rifamycin SV-Producing Amycolatopsis mediterranei U32

RifP; a membrane protein involved in rifamycin export in Amycolatopsis mediterranei

Molecular analysis and heterologous expression of the gene encoding methylmalonyl—coenzyme a mutase from rifamycin SV-producing strain Amycolatopsis mediterranei U32

Cloning and Preliminary Characterization of lh3 Gene Encoding a Putative Acetyltransferase from a Rifamycin SV-Producing Strain Amycolatopsis mediterranei

Expression in Escherichia coli, purification and kinetic analysis of the aspartokinase and aspartate semialdehyde dehydrogenase from the rifamycin SV-producing Amycolatopsis mediterranei U32

MoeA, an enzyme in the molybdopterin synthesis pathway, is required for rifamycin SV production in Amycolatopsis mediterranei U32

A preliminary study of the mechanism of nitrate-stimulated remarkable increase of rifamycin production in Amycolatopsis mediterranei U32 by RNA-seq

Improved production of rifamycin SV by Amycolatopsis mediterranei MM2 by the feeding method of carbon source

Optimization of culture medium for rifamycin SV production byAmycolatopsis mediterranei MM2 using statistical designs

A Novel Two-Component System amrB-amkB Involved in the Regulation of Central Carbohydrate Metabolism in Rifamycin SV-Producing Amycolatopsis mediterranei U32

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A Novel Two-Component System amrB-amkB Involved in the Regulation of Central Carbohydrate Metabolism in Rifamycin SV-Producing Amycolatopsis mediterranei U32

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A Novel Two-Component System amrB-amkB Involved in the Regulation of Central Carbohydrate Metabolism in Rifamycin SV-Producing Amycolatopsis mediterranei U32