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| Content Provider | Springer Nature Link |
|---|---|
| Author | Jia, Juntao Li, Zhengyi Cao, Jijuan Jiang, Yinghui Liang, Chengzhu Liu, Mengzhen |
| Copyright Year | 2013 |
| Abstract | Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cytosolic proteins were resolved by two-dimensional polyacrylamide gel electrophoresis using pH 4–7 linear gradients. Among these proteins, sixteen proteins which were strongly downregulated or upregulated in the VBNC cells were identified by MALDI-TOF-TOF mass spectrometry. The results indicated that the differentially expressed proteins were mainly focused on stress response proteins and key components of central and intermediary metabolism, like carbohydrate metabolism, transport, and translation. This study provided clues for understanding the mechanism of adaptation to the VBNC state. |
| Starting Page | 442 |
| Ending Page | 447 |
| Page Count | 6 |
| File Format | |
| ISSN | 03438651 |
| Journal | Current Microbiology |
| Volume Number | 67 |
| Issue Number | 4 |
| e-ISSN | 14320991 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2013-05-21 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Microbiology Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Applied Microbiology and Biotechnology |
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