NDLI: Isolation and Identification of a Pathogen of Silkworm Bombyx mori

Content Provider	Springer Nature Link
Author	Tao, Heng Ping Shen, Zhong Yuan Zhu, Feng Xu, Xiao Fang Tang, Xu Dong Xu, Li
Copyright Year	2010
Abstract	A pathogenic bacterial strain, ST-1, was isolated from a naturally infected silkworm. The strain was identified on the basis of its physiological and biochemical properties and the results of sequence analysis of its 16S rRNA gene. The results of the 16S rRNA gene sequence analysis revealed that ST-1 shared the highest sequence identity (more than 99%) with Pseudomonas chlororaphis subsp. aurantiaca. ST-1 bacteria were gram-negative and 0.7–0.9 × 1.3–1.5 μm long, short rods with rounded ends. The strain could utilize sodium citrate, malonate, d-glucose, sucrose, d-fructose, d-mannose, and l-arabinose. Pathogenicity of ST-1 for silkworm could be depicted as a linear regression of the logarithm (y) of ST-1 concentration against probability (x) (y = 0.4040 + 0.0600x). The median lethal concentration (LC50) was 2.12 × 104 cfu/ml. In conclusion, ST-1 was identified as Ps. chlororaphis subsp. aurantiaca. This is the first report that Ps. aurantiaca is a pathogen for silkworm Bombyx mori.
Starting Page	876
Ending Page	883
Page Count	8
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	62
Issue Number	3
e-ISSN	14320991
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-11-04
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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