NDLI: Involvement of recF in 254 nm Ultraviolet Radiation Resistance in Deinococcus radiodurans and Escherichia coli

Content Provider	Springer Nature Link
Author	Chang, Xiaosong Yang, Lan Zhao, Qing Fu, Wenjuan Chen, Hao Qiu, Zhiqun Chen, Ji an Hu, Ran Shu, Weiqun
Copyright Year	2010
Abstract	recF is a critical gene involved in the RecFOR pathway of DNA repair in Deinococcus radiodurans (D. radiodurans). To investigate the role of recF in UV-C radiation resistance, we generated the recF-deficient D. radiodurans strain R1, expressed recF in Escherichia coli (E. coli) BL21 cells, and compared the ability of each to resist UV-C radiation by F10, inactivation constant (IC), and extrapolation number (N). The mutation of recF in D. radiodurans R1 resulted in characteristic slow growth and dramatic sensitivity to UV-C irradiation. Transformation of recF into E. coli BL21 cells resulted in increased UV-C resistance compared to untransformed E. coli BL21 cells. These results suggested that recF is needed for the replication of D. radiodurans. Furthermore, as a part of the RecFOR pathway in D. radiodurans, disruption of recF could dramatically decrease the UV-C resistance of D. radiodurans and recF could increase the UV-C resistance of E. coli BL21 cells.
Starting Page	458
Ending Page	464
Page Count	7
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	61
Issue Number	5
e-ISSN	14320991
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-04-13
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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