NDLI: Analysis of Protein Expression Profiles of Halobacillus dabanensis D-8T Under Optimal and High Salinity Conditions

Content Provider	Springer Nature Link
Author	Feng, De Qin Yang, Li Fu Lu, Wei Dong Yang, Su Sheng
Copyright Year	2007
Abstract	Two-dimensional (2-D) gel electrophoresis was employed to display the expression profiles of proteins of Halobacillus dabanensis D-8T under 1%, 10%, and 20% salinities. Approximately 700 protein spots could be detected in the 2-D gels by Imagemaster™ 2D Platinum software. The molecular masses of the majority of intracellular proteins were distributed in the range of 17.5 kDa–66 kDa and isoelectric points of 4.0–5.9. In total 133 protein spots were observed with a changed expression level under different salinity conditions. Sixty-two protein spots showed upregulation and 26 new protein spots were found under high salinity conditions, while 25 protein spots were downregulated and 20 spots disappeared. Twenty-seven proteins with a markedly changed expression in hypersaline environments were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF/MS) and MASCOT. A changed expression pattern was observed for proteins related to energy-producing pathways, stress regulators, and proteins involved in the survival of strain D-8T under high salt challenges. Many proteins play necessary roles in the adaptation to high salt or as a general stress protein, and some proteins are salt-stressed specific proteins that improve the capability of salt-tolerance of strain D-8T growth under extremely hypersaline condition.
Starting Page	20
Ending Page	26
Page Count	7
File Format	PDF
ISSN	03438651
Journal	Current Microbiology
Volume Number	54
Issue Number	1
e-ISSN	14320991
Language	English
Publisher	Springer US
Publisher Date	2007-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Characterization of the Glycine Betaine Biosynthetic Genes in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T

Cloning and Characterization of the Genes for Biosynthesis of the Compatible Solute Ectoine in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T

Production and characterization of alkaline protease from Halobacillus dabanensis

Characterization of biosurfactants produced by Halobacillus dabanensis and Pontibacillus chungwhensi isolated from oil-contaminated mangrove ecosystem in Egypt

Characterization of Heterogeneous LSU rRNA Profiles in Streptomyces coelicolor Under Different Growth Stages and Conditions

GidA Expression in Salmonella is Modulated Under Certain Environmental Conditions

Expression of the Malolactic Enzyme Gene (mle) from Lactobacillus plantarum Under Winemaking Conditions

Biodegradation of Mixed Phenolic Compounds Under High Salt Conditions and Salinity Fluctuations by Arthrobacter sp. W1

Secretome Analysis of Metarhizium anisopliae Under Submerged Conditions Using Bombyx mori Chrysalis to Induce Expression of Virulence-Related Proteins

Analysis of Protein Expression Profiles of Halobacillus dabanensis D-8T Under Optimal and High Salinity Conditions

Similar Documents

Characterization of the Glycine Betaine Biosynthetic Genes in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T

Cloning and Characterization of the Genes for Biosynthesis of the Compatible Solute Ectoine in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T

Production and characterization of alkaline protease from Halobacillus dabanensis

Characterization of biosurfactants produced by Halobacillus dabanensis and Pontibacillus chungwhensi isolated from oil-contaminated mangrove ecosystem in Egypt

Characterization of Heterogeneous LSU rRNA Profiles in Streptomyces coelicolor Under Different Growth Stages and Conditions

GidA Expression in Salmonella is Modulated Under Certain Environmental Conditions

Expression of the Malolactic Enzyme Gene (mle) from Lactobacillus plantarum Under Winemaking Conditions

Biodegradation of Mixed Phenolic Compounds Under High Salt Conditions and Salinity Fluctuations by Arthrobacter sp. W1

Secretome Analysis of Metarhizium anisopliae Under Submerged Conditions Using Bombyx mori Chrysalis to Induce Expression of Virulence-Related Proteins

Analysis of Protein Expression Profiles of Halobacillus dabanensis D-8T Under Optimal and High Salinity Conditions