NDLI: Enhancement of substrate recognition ability by combinatorial mutation of β-glucosidase displayed on the yeast cell surface

Content Provider	Springer Nature Link
Author	Fukuda, Takeshi Kato Murai, Michiko Kadoso, Tetsuya Sahara, Hiroshi Hata, Yoji Suye, Shin ichiro Ueda, Mitsuyoshi
Copyright Year	2007
Abstract	Recently, in family 3 β-glucosidase (BGL), the catalytically important Asp nucleophile has been identified in the SDW segment of the SDWG sequence by site-directed mutagenesis. However, the details about the roles of each amino acid residue of the SDWG sequence have not been investigated. W293 of the SDW segment, which is the residue next to the nucleophile (D292) in family 3 BGL, is very important for hydrolytic reaction as a binder to a substrate. G294 of the SDWG sequence might play an important role in catalysis. In this study, to obtain a functional BGL1 mutant by the substitution of G294 using a genetic engineering method, the library of mutant BGL1 from Aspergillus oryzae was rapidly constructed by yeast cell surface engineering, and the hydrolytic activities of mutants were comprehensively detected. Consequently, G294F, G294W, and G294Y, in which G was substituted with aromatic amino acids, showed higher activities for substrate recognition than the parent strain (1.5-, 1.5-, and 1.6-fold, respectively). These results suggest the presence of some interaction between the sugar rings and aromatic ring of W293 at the entrance of the catalytic pocket, which enhances the substrate recognition of β-glucosidase.
Starting Page	1027
Ending Page	1033
Page Count	7
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	76
Issue Number	5
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-06-30
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Yeast cell surface engineering SDW segment Family 3 β-glucosidase Substrate recognition Comprehensive library of mutants Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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