NDLI: Comparison of CO-dependent H2 production with strong promoters in Thermococcus onnurineus NA1

Content Provider	Springer Nature Link
Author	Lee, Seong Hyuk Kim, Min Sik Bae, Seung Seob Choi, Ae Ran Lee, Jin Won Kim, Tae Wan Lee, Jung Hyun Lee, Hyun Sook Kang, Sung Gyun
Copyright Year	2013
Abstract	To overproduce biotechnologically valuable products, the expression level of target genes has been modulated by using strong promoters. In a hyperthermophilic archaeon Thermococcus onnurineus NA1, two promoters, P TN0413 and P TN0157 , which drive expression of the genes encoding the S-layer protein and glutamate dehydrogenase were inserted in front of a gene cluster encoding a carbon monoxide dehydrogenase, a hydrogenase and a Na+/H+ antiporter. Two promoters exhibited strong activity by increasing the transcription and translation levels of the gene cluster in the mutant strains by 2.5- to 49-folds and 1.4- to 3.3-folds, respectively, than the native promoter in the wild-type strain. While KS0413 with P TN0413 promoter exhibited 2.7 to 4.7 times higher transcript level than KS0157 with P TN0157 promoter, the levels of proteins were a little different between them. The biomass concentrations and H2 production rates of two mutants were 2- to 3-fold higher than those of the wild-type strain in a bioreactor where CO was supplied at a flow rate of 120 ml min−1. Two mutants showed differential response to the higher CO flow rate, 240 ml min−1, in terms of growth pattern and product formation, indicating two promoters were regulated by culture conditions. The results demonstrate that not only promoter strength but also product-forming conditions should be considered in promoter engineering.
Starting Page	979
Ending Page	986
Page Count	8
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	98
Issue Number	2
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-12-13
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Strong promoter Hyperthermophilic archaeon Carbon monoxide H2 production Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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