NDLI: Microorganism inactivation using high-pressure generation in sealed vessels under sub-zero temperature

Content Provider	Springer Nature Link
Author	Hayakawa, K. Ue, Y. Kawamura, S. Kato, T. Hayashi, R.
Copyright Year	1998
Abstract	In order to test the possibility of utilizing high pressure in bioscience and biotechnology, a simple method for high-pressure generation and its use for microbial inactivation have been studied. When a pressure vessel was filled with water, sealed tightly and cooled to sub-zero temperatures, high pressure was generated in the vessel. The pressure generation was 60 MPa at −5 °C, 103 MPa at −10 °C, and 140 MPa at −15 °C, −20 °C, and −22 °C. The high pressure generated inactivated microorganisms effectively: yeasts (Saccharomyces cerevisiae and Zygosaccharomyces rouxii), bacteria (Lactobacillus brevis and Eschericia coli), and fungi (Aspergillus niger and Aspergillus oryzae) were completely inactivated when stored in sealed vessels −20 °C for 24 h. However, Staphylococcus aureus was only partly inactivated under the same conditions. This method opens up a new application of high pressure for storing, transporting, and sterilizing of foods and biological materials.
Starting Page	415
Ending Page	418
Page Count	4
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	50
Issue Number	4
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-10-28
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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Microorganism inactivation using high-pressure generation in sealed vessels under sub-zero temperature