NDLI: Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli

Content Provider	Springer Nature Link
Author	Maric, Selma Thygesen, Mikkel B. Schiller, Jürgen Marek, Magdalena Moulin, Martine Haertlein, Michael Forsyth, V. Trevor Bogdav, Mikhail Dowhan, William Arleth, Lise Pomorski, Thomas Günther
Copyright Year	2014
Abstract	Phosphatidylcholine (PC) is a major component of eukaryotic cell membranes and one of the most commonly used phospholipids for reconstitution of membrane proteins into carrier systems such as lipid vesicles, micelles and nanodiscs. Selectively deuterated versions of this lipid have many applications, especially in structural studies using techniques such as NMR, neutron reflectivity and small-angle neutron scattering. Here we present a comprehensive study of selective deuteration of phosphatidylcholine through biosynthesis in a genetically modified strain of Escherichia coli. By carefully tuning the deuteration level in E. coli growth media and varying the deuteration of supplemented carbon sources, we show that it is possible to achieve a controlled deuteration for three distinct parts of the PC lipid molecule, namely the (a) lipid head group, (b) glycerol backbone and (c) fatty acyl tail. This biosynthetic approach paves the way for the synthesis of specifically deuterated, physiologically relevant phospholipid species which remain difficult to obtain through standard chemical synthesis.
Starting Page	241
Ending Page	254
Page Count	14
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	99
Issue Number	1
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-10-11
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Glycerophospholipids Phosphatidylcholine Selective deuteration Biosynthesis E. coli Neutron scattering NMR Mass spectrometry Evolution in the test tube Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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