NDLI: Pycnoporus laccase-mediated bioconversion of rutin to oligomers suitable for biotechnology applications

Content Provider	Springer Nature Link
Author	Uzan, Eva Portet, Bénédicte Lubra, Christian Milesi, Sandrine Favel, Anne Lesage Meessen, Laurence Lomascolo, Anne
Copyright Year	2011
Abstract	The Pycnoporus fungi are white-rot basidiomycetes listed as food- and cosmetic-grade microorganisms. Three high redox potential laccases from Pycnoporus coccineus and Pycnoporus sanguineus were tested and compared, with the commercial Suberase® as reference, for their ability to synthesise natural active oligomers from rutin (quercetin-3-rutinoside, one of the best-known naturally occurring flavonoid glycosides). The aim of this work was to develop a process with technical parameters (solvent, temperature, reaction time and raw materials) that were easy to scale up for industrial production and compatible with cosmetic and pharmaceutical formulation guidelines. The aqueous mixture of glycerol/ethanol/buffer described in this study met this requirement and allowed the solubilisation of rutin and its oxidative bioconversion into oligomers. The four flavonoid oligomer mixtures synthesised using laccases as catalysts were analysed by high performance liquid chromatography–diode array detection–negative electrospray ionisation–multistage mass spectrometry. Their chromatographic elution profiles were compared and 16 compounds were characterised and identified as dimers and trimers of rutin. The oligorutins were different in Suberase® and Pycnoporus laccase reaction mixtures. They were evaluated for their antioxidant, anti-inflammatory and anti-ageing activities on specific enzymatic targets such as cyclooxygenase (COX-2) and human matrix metalloproteinase 3 (MMP-3). Expressed in terms of IC50, the flavonoid oligomers displayed a 2.5- to 3-fold higher superoxide scavenging activity than monomeric rutin. Pycnoporus laccase and Suberase® oligorutins led to an inhibition of COX-2 of about 35% and 70%, respectively, while monomeric rutin showed a near-negligible inhibition effect, less than about 10%. The best results on MMP-3 activity were obtained with rutin oligomers from P. sanguineus IMB W006-2 laccase and Suberase® with about 70–75% inhibition.
Starting Page	97
Ending Page	105
Page Count	9
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	90
Issue Number	1
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-01-06
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Laccase Sustainable chemistry Pycnoporus Oligomerisation Rutin Biotechnology Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Peculiarities of Pycnoporus species for applications in biotechnology

Studies on the Pycnoporus sanguineus CCT-4518 laccase purified by hydrophobic interaction chromatography

Purification and characterization of laccase from Pycnoporus sanguineus and decolorization of an anthraquinone dye by the enzyme

Polyoxometalate/laccase-mediated oxidative polymerization of catechol for textile dyeing

Production of superparamagnetic nanobiocatalysts for green chemistry applications

Laccase applications in biofuels production: current status and future prospects

Critical factors affecting laccase-mediated biobleaching of pulp in paper industry

Pseudomonas: a promising biocatalyst for the bioconversion of terpenes

Laccase catalysis for the synthesis of bioactive compounds

Pycnoporus laccase-mediated bioconversion of rutin to oligomers suitable for biotechnology applications

Similar Documents

Peculiarities of Pycnoporus species for applications in biotechnology

Studies on the Pycnoporus sanguineus CCT-4518 laccase purified by hydrophobic interaction chromatography

Purification and characterization of laccase from Pycnoporus sanguineus and decolorization of an anthraquinone dye by the enzyme

Polyoxometalate/laccase-mediated oxidative polymerization of catechol for textile dyeing

Production of superparamagnetic nanobiocatalysts for green chemistry applications

Laccase applications in biofuels production: current status and future prospects

Critical factors affecting laccase-mediated biobleaching of pulp in paper industry

Pseudomonas: a promising biocatalyst for the bioconversion of terpenes

Laccase catalysis for the synthesis of bioactive compounds

Pycnoporus laccase-mediated bioconversion of rutin to oligomers suitable for biotechnology applications