NDLI: Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes

Content Provider	Springer Nature Link
Author	Mascarin, Gabriel Moura Jackson, Mark A. Behle, Robert W. Kobori, Nilce N. Júnior, Ítalo Delalibera
Copyright Year	2016
Abstract	The yeast form (blastospore) of the dimorphic insect-pathogenic fungus Beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. In this study, we evaluated the influence of two convective drying methods, various modified atmosphere packaging systems, and storage temperatures on the desiccation tolerance, storage stability, and virulence of blastospores of B. bassiana ESALQ 1432. All blastospore formulations were dried to <5 % water content equivalent to aw < 0.3. The viability of B. bassiana blastospores after air drying and spray drying was greater than 80 %. Vacuum-packaged blastospores remained viable longer when stored at 4 °C compared with 28 °C with virtually no loss in viability over 9 months regardless the drying method. When both oxygen and moisture scavengers were added to sealed packages of dried blastospore formulations stored at 28 °C, viability was significantly prolonged for both air- and spray-dried blastospores. The addition of ascorbic acid during spray drying did not improve desiccation tolerance but enhanced cell stability (∼twofold higher half-life) when stored at 28 °C. After storage for 4 months at 28 °C, air-dried blastospores produced a lower LC80 and resulted in higher mortality to whitefly nymphs (Bemisia tabaci) when compared with spray-dried blastospores. These studies identified key storage conditions (low aw and oxygen availability) that improved blastospore storage stability at 28 °C and will facilitate the commercial development of blastospores-based bioinsecticides.
Starting Page	8359
Ending Page	8370
Page Count	12
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	100
Issue Number	19
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-05-20
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Mycoinsecticides Liquid fermentation Air drying Spray drying Storage stability Oxygen scavengers Desiccants Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Glucose concentration alters dissolved oxygen levels in liquid cultures of Beauveria bassiana and affects formation and bioefficacy of blastospores

The production and uses of Beauveria bassiana as a microbial insecticide

Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor

Viability of Beauveria bassiana isolates after storage under several preservation methods

Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations

Shelf life and storage stability of spray-dried bovine colostrum powders under different storage conditions.

Sulfonylurea resistance as a new selectable marker for the entomopathogenic fungus Beauveria bassiana

Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology

Use of uridine auxotrophy (ura3) for markerless transformation of the mycoinsecticide Beauveria bassiana

Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes

Similar Documents

Glucose concentration alters dissolved oxygen levels in liquid cultures of Beauveria bassiana and affects formation and bioefficacy of blastospores

The production and uses of Beauveria bassiana as a microbial insecticide

Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor

Viability of Beauveria bassiana isolates after storage under several preservation methods

Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations

Shelf life and storage stability of spray-dried bovine colostrum powders under different storage conditions.

Sulfonylurea resistance as a new selectable marker for the entomopathogenic fungus Beauveria bassiana

Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology

Use of uridine auxotrophy (ura3) for markerless transformation of the mycoinsecticide Beauveria bassiana

Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes