NDLI: The role of autophagy in the cytotoxicity induced by recombinant human arginase in laryngeal squamous cell carcinoma

Content Provider	Springer Nature Link
Author	Lin, Chen Wang, Ziyu Li, Li He, Yong Fan, Jiajun Liu, Zhongyu Zhao, Shuwei Ju, Dianwen
Copyright Year	2015
Abstract	Laryngeal squamous cell carcinoma (LSCC), one of the most common malignant solid tumors in the world, has a high rate of mortality, recurrence, and metastasis. Recombinant human arginase (rhArg) recently has been developed in arginine deprivation therapy for a number of malignant tumors. In the present study, we observed that rhArg triggered significant cytotoxicity in human laryngeal squamous cell carcinoma Tu212 cells. Meanwhile, we observed that rhArg simultaneously activated autophagic flux in Tu212 cells, which was demonstrated by the accumulation of autophagosome and light chain 3 II (LC3-II). And, we explored the role of autophagy in cytotoxicity induced by rhArg in Tu212 cells. Autophagy inhibitors including chloroquine (CQ) and bafilomycin A1 (Baf A1) enhanced cytotoxicity induced by rhArg, implying the protective role of autophagy in rhArg-treated Tu212 cells. Moreover, Akt/mTOR signaling pathway was most possibly to participate in the rhArg-induced autophagy. Meanwhile, rhArg could upregulate the phosphorylation of ERK1/2 in a time-dependent manner. Therefore, all the results illuminated the cytoprotective role of autophagy in the treatment of rhArg in laryngeal squamous carcinoma Tu212 cells and provided a potential approach for LSCC therapy by rhArg combined with autophagy inhibitors.
Starting Page	8487
Ending Page	8494
Page Count	8
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	99
Issue Number	20
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-04-23
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Autophagy Recombinant human arginase Laryngeal squamous cell carcinoma Cytotoxicity Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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