NDLI: Production and derivate composition of trisporoids in extended fermentation of Blakeslea trispora

Content Provider	Springer Nature Link
Author	Schachtschabel, Doreen Menzel, Klaus Dieter Krauter, Gudrun David, Anja Roth, Martin Horn, Uwe Boland, Wilhelm Wöstemeyer, Johannes Schimek, Christine
Copyright Year	2010
Abstract	Trisporic acid, its precursors and derivatives are used within zygomycete fungi as communication signals and sexual regulators, and also influence the production rate of the parent compound, β-carotene. Cultivation parameters during growth and the trisporoid production phase of Blakeslea trispora were studied in two-step shake flask cultures and up-scaled fermentations. Comparison of various fermentation protocols allowed the definition of parameters governing trisporoid production. Highest yields were obtained when the initial growth phase allowed for both rapid growth and fast exhaustion of nitrogen and phosporous sources. Onset of trisporoid production is accompanied by a pH drop in the medium and triggered by nutrient limitation, nitrogen depletion being the most important factor. Supplementation of cultures with carbon at low concentration after onset of trisporoid production led to prolonged growth and higher final product accumulation. B. trispora produces trisporoids in two major series, B and C. During a first peak in trisporic acid accumulation, production of trisporic acid B exceeds that of trisporic acid C, which later accumulates at the expense of the trisporic acid B, indicating a variable regulation of the ratio between these metabolites. These data are valuable for tailoring production systems for enrichment of specific intermediates of this complex signal family.
Starting Page	241
Ending Page	249
Page Count	9
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	88
Issue Number	1
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-07-02
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Fermentation Trisporic acid Zygomycota Fungus Development Mating Nutrient starvation Blakeslea trispora Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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