NDLI: Genetic engineering of Escherichia coli to enhance production of l-tryptophan

Content Provider	Springer Nature Link
Author	Wang, Jian Cheng, Li Kun Wang, Jing Liu, Qian Shen, Tong Chen, Ning
Copyright Year	2013
Abstract	Reducing the accumulation of acetate in Escherichia coli cultures can decrease carbon efflux as by-products and reduce acetate toxicity, and therefore enable high cell density cultivation. The concentration of intracellular amino acids can be decreased by genetic modifications of the corresponding amino acid transport systems. This can increase the levels of amino acids in the fermentation broth by decreasing the feedback inhibition on the corresponding biosynthetic pathways. Here, the effects of genetic manipulation of phosphate acetyltransferase (pta), high affinity tryptophan transporter (mtr) and aromatic amino acid exporter (yddG) on l-tryptophan production were investigated. The pta mutants accumulated less acetate and showed higher capacity for producing l-tryptophan as compared with the parental strain. The strains lacking mtr, or overexpressed yddG, or with the both mtr knockout and yddG overexpression, accumulated lower concentrations of intracellular tryptophan but higher production of extracellular l-tryptophan. In the l-tryptohan fed-batch fermentation of an E. coli derived from TRTH0709/pMEL03 having deletion of pta-mtr and overexpression of yddG in a 30-L fermentor, the maximum concentration of l-tryptophan (48.68 g/L) was obtained, which represented a 15.96 % increase as compared with the parental strain. Acetate accumulated to a concentration of 0.95 g/L. The intracellular concentration of l-tryptophan was low, and the glucose conversion rate reached a high level of 21.87 %, which was increased by 15.53 % as compared with the parent strain.
Starting Page	7587
Ending Page	7596
Page Count	10
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	97
Issue Number	17
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-06-18
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Escherichia coli l-tryptophan Acetate pta mtr yddG Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Knocking out analysis of tryptophan permeases in Escherichia coli for improving L-tryptophan production

Fermentation characterization of an L-tryptophan producing Escherichia coli strain with inactivated phosphotransacetylase

Impact of deletion of the genes encoding acetate kinase on production of L-tryptophan by Escherichia coli

Gene modification of Escherichia coli and incorporation of process control to decrease acetate accumulation and increase ʟ-tryptophan production

Metabolic engineering of Escherichia coli to enhance phenylalanine production

Improvement of the production of L-tryptophan in Escherichia coli by application of a dissolved oxygen stage control strategy

Rational design and metabolic analysis of Escherichia coli for effective production of L-tryptophan at high concentration

The improved l-tryptophan production in recombinant Escherichia coli by expressing the polyhydroxybutyrate synthesis pathway

Effect of feeding strategy on l-tryptophan production by recombinant Escherichia coli

Genetic engineering of Escherichia coli to enhance production of l-tryptophan

Similar Documents

Knocking out analysis of tryptophan permeases in Escherichia coli for improving L-tryptophan production

Fermentation characterization of an L-tryptophan producing Escherichia coli strain with inactivated phosphotransacetylase

Impact of deletion of the genes encoding acetate kinase on production of L-tryptophan by Escherichia coli

Gene modification of Escherichia coli and incorporation of process control to decrease acetate accumulation and increase ʟ-tryptophan production

Metabolic engineering of Escherichia coli to enhance phenylalanine production

Improvement of the production of L-tryptophan in Escherichia coli by application of a dissolved oxygen stage control strategy

Rational design and metabolic analysis of Escherichia coli for effective production of L-tryptophan at high concentration

The improved l-tryptophan production in recombinant Escherichia coli by expressing the polyhydroxybutyrate synthesis pathway

Effect of feeding strategy on l-tryptophan production by recombinant Escherichia coli

Genetic engineering of Escherichia coli to enhance production of l-tryptophan