NDLI: Production of the fungal biocontrol agent Ulocladium atrum by submerged fermentation: accumulation of endogenous reserves and shelf-life studies

Content Provider	Springer Nature Link
Author	Frey, S. Magan, N.
Copyright Year	2001
Abstract	A method was developed for the induction of submerged conidiation of Ulocladium atrum Preuss (isolate 385) for the first time, using an oatmeal extract broth. Two inoculum types were produced by this process: spores and mycelial fragments. Spore production was stimulated by reducing the broth water potential (Ψ) to –2.1 MPa and adding 20 mM calcium chloride. In contrast, mycelial fragments were dominant at –7.0 MPa Ψ. Maximum total inoculum (mycelial fragments and conidia) yields were approximately 2×107 ml–1 after 9 days incubation at 25 °C at 100 rpm. Biomass from liquid cultures responded to water-stress by accumulating increased concentrations of endogenous sugar alcohols (polyols), particularly glycerol. Long-term shelf-life studies showed that submerged inoculum from cultures subjected to an intermediate water-stress (–2.1 MPa Ψ) and containing enhanced levels of glycerol (>300 mg g–1 freeze-dried material) retained viability significantly better (P<0.05) than that from unstressed cultures, when assessed on agar with fully available water. This level of viability was comparable to that of aerial U. atrum spores from a 4-week solid-substrate fermentation on oat grains. However, in contrast to aerial spores, the ability of submerged biomass to germinate in drier conditions declined significantly after 6 months.
Starting Page	372
Ending Page	377
Page Count	6
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	56
Issue Number	3-4
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-06-02
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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