NDLI: Nitrate regulation of α-aminoadipate reductase formation and lysine inhibition of its activity in Penicillium chrysogenum and Acremonium chrysogenum

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Nitrate regulation of α-aminoadipate reductase formation and lysine inhibition of its activity in Penicillium chrysogenum and Acremonium chrysogenum

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Nitrate regulation of α-aminoadipate reductase formation and lysine inhibition of its activity in Penicillium chrysogenum and Acremonium chrysogenum

Content Provider	Springer Nature Link
Author	Hijarrubia, M. Aparicio, J. Martín, J.
Copyright Year	2002
Abstract	α-Aminoadipate reductase (α-AAR) is a key enzyme in the branched pathway for lysine and β-lactam biosynthesis of filamentous fungi since it competes with α-aminoadipyl-cysteinyl-valine synthetase for their common substrate L-α-aminoadipic acid. The α-AAR activity in two penicillin-producing Penicillium chrysogenum strains and two cephalosporin-producing Acremonium chrysogenum strains has been studied. The α-AAR activity peaked during the growth-phase preceding the onset of antibiotic production, which coincides with a decrease in α-AAR activity, and was lower in high penicillin- or cephalosporin-producing strains. The α-AAR required NADPH for enzyme activity and could not use NADH as electron donor for reduction of the α-aminoadipate substrate. The α-AAR protein of P. chrysogenum was detected by Western blotting using anti-α-AAR antibodies. The mechanism of lysine feedback regulation in these two filamentous fungi involves inhibition of the α-AAR activity but not repression of its synthesis by lysine. This is different from the situation in yeasts where lysine feedback inhibits and represses α-AAR. Nitrate has a strong negative effect on α-AAR formation as shown by immunoblotting studies of α-AAR. The nitrate effect was reversed by lysine.
Starting Page	270
Ending Page	277
Page Count	8
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	59
Issue Number	2-3
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-05-08
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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Lysine biosynthesis inPenicillium chrysogenumis regulated by feedback inhibition of Î±-aminoadipate reductase

The thioredoxin reductase-encoding gene ActrxR1 is involved in the cephalosporin C production of Acremonium chrysogenum in methionine-supplemented medium

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Saturation mutagenesis of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase R308 site confirms its role in controlling substrate specificity

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Characterization of the lys2 gene of Acremonium chrysogenum encoding a functional α-aminoadipate activating and reducing enzyme

Direct involvement of the CreA transcription factor in penicillin biosynthesis and expression of the pcbAB gene in Penicillium chrysogenum

Nitrate regulation of α-aminoadipate reductase formation and lysine inhibition of its activity in Penicillium chrysogenum and Acremonium chrysogenum

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