NDLI: Functional maintenance and structural flexibility of microbial communities perturbed by simulated intense rainfall in a pilot-scale membrane bioreactor

Content Provider	Springer Nature Link
Author	Sato, Yuya Hori, Tomoyuki Navarro, Ronald R. Habe, Hiroshi Ogata, Atsushi
Copyright Year	2016
Abstract	Intense rainfall is one of the most serious and common natural events, causing the excessive inflow of rainwater into wastewater treatment plants. However, little is known about the impacts of rainwater dilution on the structure and function of the sludge microorganisms. Here, high-throughput sequencing of 16S ribosomal RNA (rRNA) genes was implemented to describe the microbial community dynamics during the simulated intense rainfall situation (event i) in which approximately 45 % of the sludge biomass was artificially overflowed by massive water supply in a pilot-scale membrane bioreactor. Thereafter, we investigated the functional and structural responses of the perturbed microbial communities to subsequent conditional changes, i.e., an increase in organic loading rate from 225 to 450 mg chemical oxygen demand (COD) l−1 day−1 (event ii) and an addition of a microbiota activator (event iii). Due to the event i, the COD removal declined to 78.2 %. This deterioration coincided with the decreased microbial diversity and the proliferation of the oligotrophic Aquabacterium sp. During the succeeding events ii and iii, the sludge biomass increased and the COD removal became higher (86.5–97.4 %). With the apparent recovery of the reactor performance, microbial communities became diversified and the compositions dynamically changed. Notably, various bacterial micropredators were highly enriched under the successive conditions, most likely being involved in the flexible reorganization of microbial communities. These results indicate that the activated sludge harbored functionally redundant microorganisms that were able to thrive and proliferate along with the conditional changes, thereby contributing to the functional maintenance of the membrane bioreactor.
Starting Page	6447
Ending Page	6456
Page Count	10
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	100
Issue Number	14
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-03-28
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Membrane bioreactor High-throughput sequencing Microbial community Biodiversity Resistance to disturbance Activated sludge Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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