NDLI: H2-Producing bacterial communities from a heat-treated soil inoculum

Content Provider	Springer Nature Link
Author	Iyer, Prabha Bruns, Mary Ann Zhang, Husen Ginkel, Steve Logan, Bruce E.
Copyright Year	2004
Abstract	Hydrogen gas (∼60% H2) was produced in a continuous flow bioreactor inoculated with heat-treated soil, and fed synthetic wastewater containing glucose (9.5 g l−1). The pH in the bioreactor was maintained at 5.5 to inhibit consumption of H2 by methanogens. The objective of this study was to characterize bacterial communities in the reactor operated under two different hydraulic retention times (HRTs of 30-h and 10-h) and temperatures (30show $132#°show $ 132#C and 37show $132#°show $ 132#C). At 30-h HRT, the H2 production rate was 80 ml h−1 and yield was 0.91 mol H2/mol glucose. At 10-h HRT, the H2 production rate was more than 5 times higher at 436 ml h−1, and yield was 1.61 mol H2/mol glucose. Samples were removed from the reactor under steady-state conditions for PCR-based detection of bacterial populations by ribosomal intergenic spacer analysis (RISA). Populations detected at 30-h HRT were more diverse than at 10-h HRT and included representatives of Bacillaceae, Clostridiaceae, and Enterobacteriaceae. At 10-h HRT, only Clostridiaceae were detected. When the temperature of the 10-h HRT reactor was increased from 30show $132#°show $ 132#C to 37show $132#°show $ 132#C, the steady-state H2 production rate increased slightly to 463 ml h−1 and yield was 1.8 mol H2/mol glucose. Compared to 30show $132#°show $ 132#C, RISA fingerprints at 37show $132#°show $ 132#C from the 10-h HRT bioreactor exhibited a clear shift from populations related to Clostridium acidisoli (subcluster Ic) to populations related to Clostridium acetobutylicum (subcluster Ib).
Starting Page	166
Ending Page	173
Page Count	8
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	66
Issue Number	2
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-07-29
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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