NDLI: Free fatty acid production in Escherichia coli under phosphate-limited conditions

Content Provider	Springer Nature Link
Author	Youngquist, J. Tyler Rose, Josh P. Pfleger, Brian F.
Copyright Year	2013
Abstract	Microbially synthesized fatty acids are an attractive platform for producing renewable alternatives to petrochemically derived transportation fuels and oleochemicals. Free fatty acids (FFA) are a direct precursor to many high-value compounds that can be made via biochemical and ex vivo catalytic pathways. To be competitive with current petrochemicals, flux through these pathways must be optimized to approach theoretical yields. Using a plasmid-free, FFA-producing strain of Escherichia coli, a set of chemostat experiments were conducted to gather data for FFA production under phosphate limitation. A prior study focused on carbon-limited conditions strongly implicated non-carbon limitations as a preferred media formulation for maximizing FFA yield. Here, additional data were collected to expand an established kinetic model of FFA production and identify targets for further metabolic engineering. The updated model was able to successfully predict the strain’s behavior and FFA production in a batch culture. The highest yield observed under phosphate-limiting conditions (0.1 g FFA/g glucose) was obtained at a dilution rate of 0.1 h−1, and the highest biomass-specific productivity (0.068 g FFA/gDCW/h) was observed at a dilution rate of 0.25 h−1. Phosphate limitation increased yield (∼45 %) and biomass-specific productivity (∼300 %) relative to carbon-limited cultivations using the same strain. FFA production under phosphate limitation also led to a cellular maintenance energy ∼400 % higher (0.28 g/gDCW/h) than that seen under carbon limitation.
Starting Page	5149
Ending Page	5159
Page Count	11
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	97
Issue Number	11
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-04-26
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Biofuel Chemostat Escherichia coli Fatty acid Kinetic model Thioesterase Biotechnology Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Bacterial production of free fatty acids from freshwater macroalgal cellulose

Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions

Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli

Engineering Escherichia coli for odd straight medium chain free fatty acid production

The production of ω-hydroxy palmitic acid using fatty acid metabolism and cofactor optimization in Escherichia coli

Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli

Enhancement of free fatty acid production in Saccharomyces cerevisiae by control of fatty acyl-CoA metabolism

Isobutanol production from cellobiose in Escherichia coli

Production of 2-methyl-1-butanol in engineered Escherichia coli

Free fatty acid production in Escherichia coli under phosphate-limited conditions

Similar Documents

Bacterial production of free fatty acids from freshwater macroalgal cellulose

Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions

Biosynthesis of butenoic acid through fatty acid biosynthesis pathway in Escherichia coli

Engineering Escherichia coli for odd straight medium chain free fatty acid production

The production of ω-hydroxy palmitic acid using fatty acid metabolism and cofactor optimization in Escherichia coli

Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli

Enhancement of free fatty acid production in Saccharomyces cerevisiae by control of fatty acyl-CoA metabolism

Isobutanol production from cellobiose in Escherichia coli

Production of 2-methyl-1-butanol in engineered Escherichia coli

Free fatty acid production in Escherichia coli under phosphate-limited conditions