NDLI: Carboxylesterase 2 production and characterization in human cells: new insights into enzyme oligomerization and activity

Content Provider	Springer Nature Link
Author	Lamego, Joana Cunha, Bárbara Peixoto, Cristina Sousa, Marcos F. Alves, Paula M. Simplício, Ana L. Coroadinha, Ana S.
Copyright Year	2012
Abstract	Carboxylesterase 2 (CES2), the main carboxylesterase expressed in human intestine, is an increasingly important enzyme in anti-cancer combined therapies for the treatment of different pathologies like colon adenocarcinoma and malignant glioma. The production of human recombinant CES2, in human embryonic kidney cells (HEK-293T cells) using serum-free media, is herein described. CES2 secretion to the media was achieved by the simple addition of an in-frame C-terminal 10× histidine tag (CES2-10xHis) without the need of addition of extra N-terminal signalling sequences or the mutation or deletion of the C-terminal HTEL motif responsible for retaining the protein in the lumen of endoplasmic reticulum. This secretion allowed a fourfold increase in CES2 production. The characterization of human recombinant CES2 showed that this protein exists in other active and inactive forms than the described 60 kDa monomer.
Starting Page	1161
Ending Page	1173
Page Count	13
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	97
Issue Number	3
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-03-25
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Carboxylesterases Human cells Protein expression Enzyme characterization Biotechnology Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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