NDLI: Degradation of atrazine by Frankia alni ACN14a: gene regulation, dealkylation, and dechlorination

Content Provider	Springer Nature Link
Author	Rehan, Medhat Kluge, Martin Fränzle, Stefan Kellner, Harald Ullrich, René Hofrichter, Martin
Copyright Year	2014
Abstract	Atrazine is transformed to N-isopropylammelide through hydroxyatrazine as an intermediate as indicated by high-performance liquid chromatography/mass spectroscopy in culture filtrates of Frankia alni ACN14a and Frankia sp. EuI1c. Both Frankia strains have the ability to degrade atrazine via dechlorination and dealkylation and, subsequently, may be using it as a nitrogen and carbon source as detected here by increasing their growth patterns. Bioinformatic analysis of the Frankia genomes revealed that a potential gene cluster involved in atrazine decomposition contains three genes, namely, trzN (FRAAL1474 and FraEuI1c_5874), atzB (FRAAL1473 and FraEuI1c_5875), and atzR (FRAAL1471). The relative messenger RNA gene expression of the former genes was examined by qRT-PCR. The LysR-type transcriptional regulator atzR (FRAAL1471), which is expected to control the cluster expression, showed a 13-fold increase in the expression level under atrazine stress. Moreover, the putative adenosine aminohydrolase 3 atzB (FRAAL1473), which is expected to dealkylate the N-ethyl group of atrazine, showed also an increased expression by factor 16 with increased exposure. Eventually, the trzN (FRAAL1474) gene, which is predicted to encode a putative amidohydrolase catalyzing atrazine dechlorination, exhibited 31-fold increased expression. To our best knowledge, this is the first report about adenosine aminohydrolase 3 function in the dealkylation of the N-ethyl group from atrazine.
Starting Page	6125
Ending Page	6135
Page Count	11
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	98
Issue Number	13
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-03-28
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Frankia Actinorhizal symbiosis s-Triazine biodegradation qRT-PCR Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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