NDLI: Enhanced rifamycin SV production by submerged fermentation using Amycolatopsis mediterranei

Content Provider	Springer Nature Link
Author	Nagavalli, M. Ponamgi, S. P. D. Girijashankar, V. Rao, L. Venkateswar
Copyright Year	2015
Abstract	Rifamycin is a broad-spectrum antimicrobial drug produced commercially by submerged fermentation where the yields are far less in comparison to its demand in human drug therapy. Addressing the need, sequential mutational strain improvement was carried using UV and EtBr that resulted in improved strain yielding rifamycin SV up to 4.32 g/L. Further optimization of six important fermentation factors was followed which include temperature, agitation, inoculum level, period of fermentation, inorganic nitrogen source and amino acids. For the first time, we report a maximum yield of 5.32 g/L of rifamycin SV. Among the amino acids, proline known for its slowest assimilation by Amycolatopsis mediterranei produced the highest improvement in antibiotic yields. Following mutational strain improvement and process optimization, a total of 3.8-fold increase in antibiotic titre was achieved. Following a conventional procedure of mutational strain improvement, highest yield of rifamycin SV was reported by optimizing submerged fermentation process.
Starting Page	7505
Ending Page	7513
Page Count	9
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	99
Issue Number	18
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-05-31
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Rifamycin SV Amycolatopsis mediterranei Mutational strain improvement Submerged fermentation optimization Proline Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Improved production of rifamycin SV by Amycolatopsis mediterranei MM2 by the feeding method of carbon source

Optimization of culture medium for rifamycin SV production byAmycolatopsis mediterranei MM2 using statistical designs

Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. I. The role of colony morphology and nitrogen sources in productivity

Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. III. Production in fed-batch mode in shake flasks

Improvement of industry-applied rifamycin B-producing strain, Amycolatopsis mediterranei, by rational screening

A preliminary study of the mechanism of nitrate-stimulated remarkable increase of rifamycin production in Amycolatopsis mediterranei U32 by RNA-seq

Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. IV. Production in the fermentor

Cloning and Preliminary Characterization of lh3 Gene Encoding a Putative Acetyltransferase from a Rifamycin SV-Producing Strain Amycolatopsis mediterranei

MoeA, an enzyme in the molybdopterin synthesis pathway, is required for rifamycin SV production in Amycolatopsis mediterranei U32

Enhanced rifamycin SV production by submerged fermentation using Amycolatopsis mediterranei

Similar Documents

Improved production of rifamycin SV by Amycolatopsis mediterranei MM2 by the feeding method of carbon source

Optimization of culture medium for rifamycin SV production byAmycolatopsis mediterranei MM2 using statistical designs

Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. I. The role of colony morphology and nitrogen sources in productivity

Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. III. Production in fed-batch mode in shake flasks

Improvement of industry-applied rifamycin B-producing strain, Amycolatopsis mediterranei, by rational screening

A preliminary study of the mechanism of nitrate-stimulated remarkable increase of rifamycin production in Amycolatopsis mediterranei U32 by RNA-seq

Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. IV. Production in the fermentor

Cloning and Preliminary Characterization of lh3 Gene Encoding a Putative Acetyltransferase from a Rifamycin SV-Producing Strain Amycolatopsis mediterranei

MoeA, an enzyme in the molybdopterin synthesis pathway, is required for rifamycin SV production in Amycolatopsis mediterranei U32

Enhanced rifamycin SV production by submerged fermentation using Amycolatopsis mediterranei