NDLI: Expression of serotonin derivative synthetic genes on a single self-processing polypeptide and the production of serotonin derivatives in microbes

Content Provider	Springer Nature Link
Author	Park, Munyoung Kang, Kiyoon Park, Sangkyu Kim, Young Soon Ha, Sun Hwa Lee, Shin Woo Ahn, Mi Jeong Bae, Jung Myung Back, Kyoungwhan
Copyright Year	2008
Abstract	The plant-specific serotonin derivatives feruloylserotonin (FS) and 4-coumaroylserotonin (CS) are synthesized by the enzymes 4-coumarate:coenzyme A ligase (4CL) and serotonin N-hydroxycinnamoyltransferase (SHT). To express these genes coordinately, SHT was fused in-frame with the self-processing FDMV 2A sequence followed by 4CL in a single open reading frame and introduced into Escherichia coli or Saccharomyces cerevisiae. The transgenes were abundantly expressed in both recombinant microbes, but functional expression was achieved only in yeast, with cleavage at the 2A sequence yielding monomeric SHT-2A and 4CL as judged by immunoblot and product analyses. In the presence of an exogenous supply of precursors such as serotonin and ferulic acid, the recombinant yeast synthesized 4.5 mg l−1 FS in the medium while 0.02 mg l−1 FS was produced in the cells. Time-course analysis indicated peak accumulation of FS at 24 h after induction, and this level was maintained until 96 h. The optimum precursor concentration was 2 mM. A series of serotonin derivatives was produced by adding various cinnamate derivative precursors with serotonin; 2.5 mg l−1 caffeoylserotonin (CaS) and 1.4 mg l−1 CS were produced, whereas no sinapoylserotonin or cinnamoylserotonin was yielded.
Starting Page	43
Ending Page	49
Page Count	7
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	81
Issue Number	1
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-08-16
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Serotonin derivatives 2A sequence of foot-and-mouth disease virus Co-ordinate expression Feruloylserotonin Escherichia coli Saccharomyces cerevisiae Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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