NDLI: Engineering of an l-arabinose metabolic pathway in Corynebacterium glutamicum

Content Provider	Springer Nature Link
Author	Kawaguchi, Hideo Sasaki, Miho Vertès, Alain A. Inui, Masayuki Yukawa, Hideaki
Copyright Year	2007
Abstract	Corynebacterium glutamicum was metabolically engineered to broaden its substrate utilization range to include the pentose sugar l-arabinose, a product of the degradation of lignocellulosic biomass. The resultant CRA1 recombinant strain expressed the Escherichia coli genes araA, araB, and araD encoding l-arabinose isomerase, l-ribulokinase, and l-ribulose-5-phosphate 4-epimerase, respectively, under the control of a constitutive promoter. Unlike the wild-type strain, CRA1 was able to grow on mineral salts medium containing l-arabinose as the sole carbon and energy source. The three cloned genes were expressed to the same levels whether cells were cultured in the presence of d-glucose or l-arabinose. Under oxygen deprivation and with l-arabinose as the sole carbon and energy source, strain CRA1 carbon flow was redirected to produce up to 40, 37, and 11%, respectively, of the theoretical yields of succinic, lactic, and acetic acids. Using a sugar mixture containing 5% d-glucose and 1% l-arabinose under oxygen deprivation, CRA1 cells metabolized l-arabinose at a constant rate, resulting in combined organic acids yield based on the amount of sugar mixture consumed after d-glucose depletion (83%) that was comparable to that before d-glucose depletion (89%). Strain CRA1 is, therefore, able to utilize l-arabinose as a substrate for organic acid production even in the presence of d-glucose.
Starting Page	1053
Ending Page	1062
Page Count	10
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	77
Issue Number	5
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-10-27
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Corynebacterium glutamicum l-arabinose Lignocellulosic biomass Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Engineering of pentose transport in Corynebacterium glutamicum to improve simultaneous utilization of mixed sugars

Corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products

Carbohydrate metabolism in Corynebacterium glutamicum and applications for the metabolic engineering of l-lysine production strains

Putrescine production by engineered Corynebacterium glutamicum

Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis byCorynebacterium glutamicum

Engineering of an L-arabinose metabolic pathway in Rhodococcus jostii RHA1 for biofuel production

Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum

Metabolic activity of Corynebacterium glutamicum grown on l-lactic acid under stress

Metabolic engineering of 1,2-propanediol pathways in Corynebacterium glutamicum

Engineering of an l-arabinose metabolic pathway in Corynebacterium glutamicum

Similar Documents

Engineering of pentose transport in Corynebacterium glutamicum to improve simultaneous utilization of mixed sugars

Corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products

Carbohydrate metabolism in Corynebacterium glutamicum and applications for the metabolic engineering of l-lysine production strains

Putrescine production by engineered Corynebacterium glutamicum

Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis byCorynebacterium glutamicum

Engineering of an L-arabinose metabolic pathway in Rhodococcus jostii RHA1 for biofuel production

Amino acid production from rice straw and wheat bran hydrolysates by recombinant pentose-utilizing Corynebacterium glutamicum

Metabolic activity of Corynebacterium glutamicum grown on l-lactic acid under stress

Metabolic engineering of 1,2-propanediol pathways in Corynebacterium glutamicum

Engineering of an l-arabinose metabolic pathway in Corynebacterium glutamicum