NDLI: Synthetic operon for (R,R)-2,3-butanediol production in Bacillus subtilis and Escherichia coli

Content Provider	Springer Nature Link
Author	Oliveira, Rafael R. Nicholson, Wayne L.
Copyright Year	2015
Abstract	To reduce dependence on petroleum, an alternative route to production of the chemical feedstock 2,3-butanediol (2,3-BD) from renewable lignocellulosic sources is desirable. In this communication, the genes encoding the pathway from pyruvate to 2,3-BD (alsS, alsD, and bdhA encoding acetolactate synthase, acetolactate decarboxylase, and butanediol dehydrogenase, respectively) from Bacillus subtilis were engineered into a single tricistronic operon under control of the isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible Pspac promoter in a shuttle plasmid capable of replication and expression in either B. subtilis or Escherichia coli. We describe the construction and performance of a shuttle plasmid carrying the IPTG-inducible synthetic operon alsSDbdhA coding for 2,3-BD pathway capable of (i) expression in two important representative model microorganisms, the gram-positive B. subtilis and the gram-negative E. coli; (ii) increasing 2,3-BD production in B. subtilis; and (iii) successfully introducing the B. subtilis 2,3-BD pathway into E. coli. The synthetic alsSDbdhA operon constructed using B. subtilis native genes not only increased the 2,3-BD production in its native host but also efficiently expressed the pathway in the heterologous organism E. coli. Construction of an efficient shuttle plasmid will allow investigation of 2,3-BD production performance in related organisms with industrial potential for production of bio-based chemicals.
Starting Page	719
Ending Page	728
Page Count	10
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	100
Issue Number	2
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-10-10
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	2,3-Butanediol Bacillus subtilis Butanediol dehydrogenase Escherichia coli Shuttle plasmid Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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Constructing a synthetic constitutive metabolic pathway in Escherichia coli for (R, R)-2,3-butanediol production

Enhanced production of 2,3-butanediol by engineered Bacillus subtilis

Effects of genetic modifications and fermentation conditions on 2,3-butanediol production by alkaliphilic Bacillus subtilis

The LysR-type transcriptional regulator (LTTR) AlsR indirectly regulates expression of the Bacillus subtilis bdhA gene encoding 2,3-butanediol dehydrogenase

2,3-Butanediol production from cellobiose using exogenous beta-glucosidase-expressing Bacillus subtilis

Microbial production of meso-2,3-butanediol by metabolically engineered Escherichia coli under low oxygen condition

Screening of novel bacteria for the 2,3-butanediol production

Stereospecificity of Corynebacterium glutamicum 2,3-butanediol dehydrogenase and implications for the stereochemical purity of bioproduced 2,3-butanediol

Deletion of lactate dehydrogenase in Enterobacter aerogenes to enhance 2,3-butanediol production

Synthetic operon for (R,R)-2,3-butanediol production in Bacillus subtilis and Escherichia coli

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