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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ramos, Ana Coesel, Sacha Marques, Ana Rodrigues, Marta Baumgartner, Alexandra ronha, João Rauter, Amélia Brenig, Bertram Varela, João |
| Copyright Year | 2008 |
| Abstract | The halotolerant green alga Dunaliella salina accumulates large amounts of β-carotene when exposed to various stress conditions. Although several studies concerning accumulation and biotechnological production of β-carotene have been published, the molecular basis and regulation of the genes involved in carotenoid biosynthesis in D. salina are still poorly known. In this paper, we report the isolation and regulation of the lycopene β-cyclase (Lcy-β) gene by abiotic stress. The function of this gene was determined by heterologous genetic complementation in E. coli. Gene expression and physiological analyses revealed that D. salina Lcy-β steady-state transcript and carotenoid levels were up-regulated in response to all stress conditions tested (salt, light and nutrient depletion). The results presented here suggest that nutrient availability is a key factor influencing carotenogenesis as well as carotenoid biosynthesis-related gene expression in D. salina. |
| Starting Page | 819 |
| Ending Page | 828 |
| Page Count | 10 |
| File Format | |
| ISSN | 01757598 |
| Journal | Applied Microbiology and Biotechnology |
| Volume Number | 79 |
| Issue Number | 5 |
| e-ISSN | 14320614 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2008-05-07 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Carotenoid biosynthesis Dunaliella salina Lycopene β-cyclase Gene expression Stress response regulation Microbial Genetics and Genomics Microbiology Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Applied Microbiology and Biotechnology Biotechnology |
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