NDLI: Monitoring Arthrobacter protophormiae RKJ100 in a ‘tag and chase’ method during p-nitrophenol bio-remediation in soil microcosms

Content Provider	Springer Nature Link
Author	Pandey, Gunjan Pandey, Janmejay Jain, Rakesh K.
Copyright Year	2005
Abstract	Monitoring of micro-organisms released deliberately into the environment is essential to assess their movement during the bio-remediation process. During the last few years, DNA-based genetic methods have emerged as the preferred method for such monitoring; however, their use is restricted in cases where organisms used for bio-remediation are not well characterized or where the public domain databases do not provide sufficient information regarding their sequence. For monitoring of such micro-organisms, alternate approaches have to be undertaken. In this study, we have specifically monitored a p-nitrophenol (PNP)-degrading organism, Arthrobacter protophormiae RKJ100, using molecular methods during PNP degradation in soil microcosm. Cells were tagged with a transposon-based foreign DNA sequence prior to their introduction into PNP-contaminated microcosms. Later, this artificially introduced DNA sequence was PCR-amplified to distinguish the bio-augmented organism from the indigenous microflora during PNP bio-remediation.
Starting Page	757
Ending Page	760
Page Count	4
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	70
Issue Number	6
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-09-17
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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