NDLI: Increase of organic solvent tolerance by overexpression of manXYZ in Escherichia coli

Content Provider	Springer Nature Link
Author	Okochi, Mina Kurimoto, Masaki Shimizu, Kazuri Honda, Hiroyuki
Copyright Year	2006
Abstract	Transcriptional analysis has been investigated to detect the genes involved in organic solvent tolerance. A time course of gene expression profiles of Escherichia coli after exposure to organic solvents revealed that the expression levels of manX, manY, and manZ genes were strongly upregulated by 13.2-, 10.0-, and 7.0-folds, respectively, after 2 min and then decreased after 10 min. Organic solvent tolerance of E. coli was investigated by inducing overexpression of manX, manY, and manZ genes and manXYZ operon that encode a sugar transporter of the phosphotransferase system. Although the expression of manX, manY, and manZ alone was not effective, the organic solvent tolerance level was increased by the expression of manXYZ. The intracellular hexane level was kept lower in E. coli cells overexpressing manXYZ after 4 h of incubation with hexane.
Starting Page	1394
Ending Page	1399
Page Count	6
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	73
Issue Number	6
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-10-20
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Organic solvent tolerance DNA microarray manXYZ Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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Increase of organic solvent tolerance by overexpression of manXYZ in Escherichia coli