NDLI: Impact of each individual component of the mutated PTSNag on glucose uptake and phosphorylation in Ralstonia eutropha G+1

Content Provider	Springer Nature Link
Author	Raberg, Matthias Kaddor, Chlud Kusian, Bernhard Stahlhut, Gertrud Budiva, Radka Kolev, Nikola Bowien, Botho Steinbüchel, Alexander
Copyright Year	2012
Abstract	A recent study of the UV-generated glucose-utilizing mutant Ralstonia eutropha G+1 comprising transcriptomic and proteomic analyses revealed clear evidence that glucose is transported by the N-acetylglucosamine-specific phosphotransferase system (PTSNag), which is overexpressed in this mutant due to a derepression of the encoding nag operon by an identified insertion mutation in nagR (Raberg et al., Appl Environ Microbiol 77:2058–2070, 2011). The inability of the defined deletion mutant R. eutropha G+1∆nagFEC to utilize glucose confirms this finding. Furthermore, a missense mutation in nagE (membrane component comprising the cell membrane spanning EIICNag and the cytosolic domain EIIBNag) was identified, which yields a substitution of an alanine by threonine at aa 153 of NagE and may affect glucose specificity of the mutated PTSNag in R. eutropha G+1. The investigation of various generated deletion and substitution mutants of R. eutropha H16 and G+1 in this study was able to elucidate these phenomena. It could be shown that the porin NagC, encoded by nagC being part of the nag operon, is not necessary, while NagE is required and is probably responsible for glucose transport through the cell membrane. The intracellular phosphorylation of glucose is obviously mediated by the glucokinase GLK and not by NagF (cytosolic component comprising the three soluble domains EIIANag, HPrNag, and EINag). Our data clearly indicate that the derepression of the nag operon is essential for glucose uptake. The point mutation in NagE is not an essential prerequisite for glucose transport although it increased glucose transport as observed in this study.
Starting Page	735
Ending Page	744
Page Count	10
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	95
Issue Number	3
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-02-04
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Ralstonia eutropha Glucose utilization PHB Carbohydrate phosphotransferase system (PTS) nag operon Microbiology Biotechnology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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